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目的：建立简便、快速、灵敏的精氨酸测定方法。方法：采用阴极化二阶导数单扫描示波极谱法测定药品中精氨酸含量。结果：精氨酸在－１．４０Ｖ处有一灵敏极谱峰，峰电流与精氨酸在１．０×１０－４～１．０×１０－３ｍｏｌ／Ｌ之间呈线性关系，检测限为６．２５×１０－５ｍｏｌ／Ｌ，回归方程Ｙ＝５．５０Ｘ－２．８６１５，相关系数ｒ＝０．９９９３，对样品测定结果表明，平均标准差为０．０８μｍｏｌ，平均变异系数ＣＶ为２．２３％，样品加标回收率范围在９８．５％～９８．６％。结论：本法具有重现性好、特异性强、准确度高、操作简便、快速的特点，适用于药品等物质中精氨酸的测定
Objective: To establish a simple, rapid and sensitive method for the determination of arginine. Methods: Determination of arginine in drugs by single-sweep oscillopolarography with second-order derivative of cathodic polarization. Results: Arginine showed a polarographic peak at -1.40V. The peak current was linear with arginine concentration of 1.0 × 10-4 ~ 1.0 × 10-3mol / L. The detection limit was 6.25 × 10-5mol / L, the regression equation Y = 5.50X-2.8615, the correlation coefficient r = 0.9993, the results of sample determination showed that the average standard deviation of 0.08μmol, the average CV of 2 .23%. The spiked recoveries ranged from 98.5% to 98.6%. Conclusion: This method has the characteristics of good reproducibility, specificity, high accuracy, easy operation, fast, suitable for the determination of arginine in drugs and other substances