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作者通过体外骨髓半固体和长期液体培养（ＬＴＢＭＣ），研究了干扰素－ａ（ＩＦＮ－ａ）对慢性粒细胞白血病（ＣＭＬ）细胞的作用。在半固体琼脂培养体系中，ＩＦＮ－ａ能抑制正常对照和ＣＭＬ患者７天和１０天ＣＦＵ－ＧＭ，而不抑制１４天及其后的ＣＦＵ－ＧＭ。在ＬＴＢＭＣ中，于培养开始只加一次ＩＦＮ－ａ（１０￣２Ｕ／ｍｌ，１０￣３Ｕ／ｍｌ，１０￣４Ｕ／ｍｌ），对非贴壁层细胞数和基质细胞层的形成均无明显影响，但大剂量ＩＦＮ－ａ（１０￣３Ｕ／ｍｌ和１０￣４Ｕ／ｍｌ组）对非贴壁层的ＣＦＵ－ＧＭ抑制明显；每周连续加用ＩＦＮ－ａ（１０Ｕ／ｍｌ，１０￣２Ｕ／ｍｌ，１０￣３Ｕ／ｍｌ），对非贴壁层细胞数和ＣＦＵ－ＧＭ均有抑制作用，而且ＩＦＮ－ａ浓度愈高，贴壁层的形成愈少。上述结果表明：ＩＦＮ－ａ优先抑制晚期ＣＦＵ－ＧＭ，对基质细胞也有一定抑制作用。
The authors investigated the effect of interferon-a (IFN-a) on chronic myeloid leukemia (CML) cells by in vitro bone marrow semi-solid and long-term liquid culture (LTBMC). In a semi-solid agar culture system, IFN-α inhibited CFU-GM at 7 days and 10 days in normal controls and CML patients without inhibiting CFU-GM at day 14 and thereafter. In LTBMC, IFN-α (10 ~ 2U / ml, 10 ~ 3U / ml, 10 ~ 4U / ml) added at the beginning of culture showed no significant effect on the number of non-adherent cells and the formation of stromal cells (10 ~ 3U / ml and 10 ~ 4U / ml group) on the non-adherent layer of CFU-GM significantly inhibited; weekly continuous addition of IFN-a (10U / ml, ml, 10 ~ 3U / ml) on the number of non-adherent cells and CFU-GM were inhibited, and the higher the concentration of IFN-a, the formation of adherent layer less. The above results show that: IFN-a preferentially inhibits late CFU-GM, also has some inhibition of stromal cells.