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目的监测山东省2003-2004年汉坦病毒(HV)宿主动物感染状况,为制定防治措施提供依据。方法采用夹夜法捕捉HV宿主动物,液氮保存,用直接荧光法检测宿主动物肺抗原,间接荧光检测血清抗体。结果宿主动物HV带毒率分别为褐家鼠6.24%,小家鼠2.55%,黑线姬鼠5.00%,鼠句鼠青5.56%,其余鼠种未发现带毒,核酸分析均为汉城型HV;室内以褐家鼠和小家鼠为优势鼠种,野外以褐家鼠、黑线姬鼠和大仓鼠为优势鼠种;鼠带毒率与人间疫情间存在着明显的相关关系(r=0.920)。结论山东省HV宿主以褐家鼠和小家鼠为主。
Objective To monitor the infection status of Hantaan virus (HV) host animal in Shandong Province from 2003 to 2004, and provide evidence for the establishment of control measures. Methods The host animal was captured by the nocturnal method and stored in liquid nitrogen. The lung antigens of the host animals were detected by direct fluorescence assay and the serum antibodies were detected by indirect fluorescence. Results The HV virulence rates of host animals were 6.24% for Rattus norvegicus, 2.55% for Mus musculus, 5.00% for Apodemus agrarius and 5.56% for Eupolyphaga muricatus, respectively. ; Rattus norvegicus and Mus musculus were the dominant species indoors, and Rattus norvegicus, Apodemus agrarius and Hamster were the dominant species in the field. There was a significant correlation between the virulent rate and the human epidemic (r = 0.920). Conclusion The hosts of HV in Shandong Province are mainly Rattus norvegicus and Mus musculus.