论文部分内容阅读
目的了解浙江省宁波市人乳头瘤病毒16型(Human Papillomavirus Type16,HPV16)的分布和L1区基因变异情况。方法收集宁波市社区和某医院就诊妇女的宫颈脱落细胞样本进行HPV分型检测,并对其中HPV16阳性株进行基因序列分析,并预测蛋白疏水性及抗原性。结果在778例社区人群样本中HPV16阳性23例,检出率3.0%,在1 848例就诊人群样本中HPV16阳性87例,检出率4.7%;在HPV16阳性标本中随机抽取29例进行L1基因扩增,阳性率为55.2%(16/29),经测序获得完整序列13条,L1区有12处基因变异,其中C6240G、A6432G、6902插入ATC和6950缺失TGA为主要突变形式(13/13);系统进化树分析显示,宁波市HPV16存在亚洲型和欧洲型;和参考株相比,其蛋白疏水性和抗原性在氨基酸289~292位减弱、448~449位增强、450~465位减弱。结论宁波社区人群HPV16感染率较高,多数感染者携带病毒的L1基因发生变异,这些突变引起HPV16 L1蛋白疏水性和抗原性改变。
Objective To investigate the distribution of human papillomavirus type 16 (HPV16) and the variation of L1 gene in Ningbo City, Zhejiang Province. Methods Cervical exfoliated cells were collected from Ningbo community and a hospital for HPV genotyping. The HPV16 positive strains were sequenced and the protein hydrophobicity and antigenicity were predicted. Results The positive rate of HPV16 was 23% in 778 community samples, and the detection rate was 3.0%. Among the 1848 samples, 87 were positive for HPV16, and the detection rate was 4.7%. Among the HPV16 positive samples, 29 were randomly selected for L1 gene The positive rate was 55.2% (16/29). After sequencing, 13 complete sequences were obtained. There were 12 genetic variations in L1 region. Among them, C6240G, A6432G and 6902 were inserted into ATC and 6950 deletion TGA was the main mutation (13/13 ). Phylogenetic tree analysis showed that HPV16 existed in Asia and Europe in Ningbo. Compared with the reference strain, the protein hydrophobicity and antigenicity of HPV16 were weakened at amino acids 289-292, 448-449 and 450-565 . Conclusion The prevalence of HPV16 infection is high in Ningbo community. The L1 gene of most of the infected patients mutated. These mutations caused the changes of HPV16 L1 protein hydrophobicity and antigenicity.