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目的探讨过表达CT120B基因对肺腺癌细胞生长的影响及其诱导的基因表达谱改变。方法用cDNA转染方法建立稳定过表达CT120B细胞株;CCK-8方法和裸鼠致瘤实验观察过表达CT120B对细胞在体外和体内生长的影响;表达阵列分析显示过表达CT120B诱导的基因表达谱变化;流式细胞仪检测细胞周期分布和细胞凋亡。结果过表达CT120B的SPC-A-1细胞株生长速度明显减慢,在裸鼠体内致瘤能力降低。在稳定过表达CT120B的细胞中,cyclin E1、cdk 2、c-kit和 CXCR4等34个基因表达下调,caspase 8等4个基因表达上调。过表达CT120B诱导细胞G1期停滞, 对细胞凋亡无明显影响。结论过表达CT120B通过诱导G1期停滞抑制肺腺癌细胞生长,稳定过表达CT120B细胞株中,c-kit和CXCR4等基因表达下调也有助于CT120B的生长抑制活性。
Objective To investigate the effect of over-expressed CT120B gene on the growth of lung adenocarcinoma cells and the change of its gene expression profile. Methods CT120B cells were stably overexpressed by cDNA transfection. CCK-8 and tumorigenicity in nude mice were used to observe the effects of over-expressed CT120B on cell growth in vitro and in vivo. The expression of CT120B induced by gene expression profiling Changes; Cell cycle distribution and apoptosis were detected by flow cytometry. Results The growth of SPC-A-1 cells over-expressing CT120B significantly slowed down and the tumorigenicity decreased in nude mice. In the cells stably overexpressing CT120B, 34 genes such as cyclin E1, cdk 2, c-kit and CXCR4 were down-regulated while 4 genes such as caspase 8 were up-regulated. Overexpression of CT120B induced cell G1 arrest and had no significant effect on apoptosis. Conclusion Overexpression of CT120B can inhibit the growth of human lung adenocarcinoma cells by inducing G1 phase arrest. Down-regulation of c-kit and CXCR4 gene expression in CT120B cells stably overexpression also contributes to the growth inhibitory activity of CT120B.