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Primary rat hepatocytes were cultured using different in vitro models and the enzym e leakage,albumin secretion,and cytochrome P4 5 0 1A(CYP1A) activity were observed.The re- sults showed that the level of L DH was decreased over time in culture.However,on day5 ,L DH showed a significant increase in monolayer culture (MC) while after day 8no L DH was detectable in sandwich culture (SC) . The levels of AST and AL T did not change significantly over the inves- tigated time. The CYP 1A activity was gradually decreased in a time- dependent manner in MC and SC.The decline of CYP1A was faster in MC than in SC.This effectwas partially reversed by us- ing cytochrom e P4 5 0 (CYP4 5 0 ) inducer such as Omeprazol and3- m ethylcholanthrene(3- MC) and the CYP1A induction was always higher in MC than in SC.In bioreactor basic CYP1A activity was preserved over2 weeks and the highest album in production was observed in bioreactor fol- lowed by SC and MC. Taken together,it was indicated each investigated model had its advantages and disadvantages. It was also underlined thatvarious in vitro models may address different ques- tions.
Primary rat hepatocytes were cultured using different in vitro models and the enzym e leakage, albumin secretion, and cytochrome P4 5 0 1A (CYP1A) activity were observed. The re- sults showed that the level of L DH was decreased over time in culture. However, on day 5, L DH showed a significant increase in monolayer culture (MC) while after day 8 no L DH was detectable in sandwich culture (SC). The levels of AST and AL T did not change significantly over the inves- tigated time. The CYP 1A activity was gradually decreased in a time-dependent manner in MC and SC. Decline of CYP1A was faster in MC than in SC.This effectwas than reversed by CYPLING CYCLIC P450 (CYP450) inducer such as Omeprazol and 3-m ethylcholanthrene (3-MC) and the CYP1A induction was always higher in MC than in SC. In bioreactor basic CYP1A activity was preserved over 2 weeks and the highest album in production was observed in bioreactor followed by SC and MC Taken together, it was indicated each investigated model had its advantages and disadvantages. It was also underlined thatvarious in vitro models may address different ques tions.