论文部分内容阅读
目的 研究血管内皮生长因子(VEGF)在增殖性玻璃体视网膜病变(PVR)玻璃体中的表达,分析VEGF在PVR中的作用。方法 本文共有PVR37例,其中视网膜脱离PVR22例,外伤性PVR15例。以12例突然死亡的无眼部疾病的成年人作为正常对照。采用双抗夹心法(ELISA)检测病例组、对照组玻璃体 VECF水平。结果 37例 PVR玻璃体 VEGF220.52±101.97pg/ml,其中 22例网脱PVR玻璃体VEGF238.90±61.24pg/ml,外伤性PVR玻璃体VEGF193.58±58.17pg/ml。正常对照组玻璃体 VEGF89.90±36.46pg/ml。PVR玻璃体 VECF水平明显高于正常对照组(P<0.05)。VEGF 在PVRA级、B级水平较高,且随PVR 程度增加而降低。有危险因素PVR的玻璃体VEGF 水平高于无危险因素 PVR。结论 作为细胞间的传导信号,VEGF参与PVR的发生发展。VEGF在PVR 中呈上行性表达。VEGF 不仅在缺血性眼部疾病中呈高表达,而且在某些非缺血性眼部疾病中表达升高。VEGF 主要在 PVR早期发挥作用,PVR 危险因素可以刺激玻璃体 VEGF上行性表达。
Objective To investigate the expression of vascular endothelial growth factor (VEGF) in the vitreous of proliferative vitreoretinopathy (PVR) and analyze the role of VEGF in PVR. MethodsThere are 37 PVR cases, including 22 cases of retinal detachment from PVR and 15 cases of traumatic PVR. 12 cases of sudden death without eye diseases in adults as a normal control. The level of vitreous VECF in case group and control group was detected by double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Results 37 cases of PVR vitreous body VEGF220.52 ± 101.97pg / ml, including 22 cases of reticular PVR vitreous VEGF238.90 ± 61.24pg / ml, traumatic PVR vitreous VEGF193.58 ± 58.17pg / ml. The normal control group vitreous VEGF89.90 ± 36.46pg / ml. PVR vitreous body VECF levels were significantly higher than the normal control group (P <0.05). VEGF is at the PVRA level, with a higher level of B and a lower level of PVR. Vitreous VEGF levels were higher in risk PVR than in risk-free PVR. Conclusion As a signaling signal between cells, VEGF is involved in the development of PVR. VEGF is up-regulated in PVR. VEGF is not only highly expressed in ischemic eye diseases, but also elevated in some non-ischemic eye diseases. VEGF mainly plays an important role in the early PVR, and PVR risk factors can stimulate the up-regulated expression of vitreous VEGF.