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目的探讨丹参注射液对冻融小鼠卵巢同种异体移植早期光镜结构及超微结构的影响。方法收集1日龄小鼠卵巢,慢冻速融后移植至F1代8～12周雄鼠的肾被膜下,分别于移植后第2天(2d)、第7天(7d)和第14天(14d)回收两组卵巢移植物。光镜和电镜技术观察卵巢组织冻融前后和移植前后的组织形态学改变、计数卵泡数量。结果小鼠卵巢经冻融后原始卵泡的存活率为87.9%。光、电镜观察卵巢组织移植后7d卵泡细胞和颗粒细胞结构欠完整,移植后14d卵泡结构恢复正常。移植后14d丹参组各级卵泡数及卵泡存活率(30.1%)多于生理盐水组(13.2%),差异显著(P<0.05)。结论结合光镜和电镜观察能更全面地评价冻融过程和移植过程对小鼠卵巢组织的影响,移植早期的缺血缺氧对小鼠卵巢组织造成了远比冻融过程更严重的损伤;丹参注射液可能促进移植后冻融小鼠卵泡发育,减少细胞凋亡。
Objective To investigate the effect of Salvia Miltiorrhiza injection on the light microscopy and ultrastructure of the ovariectomized mice during the early stage of freezing-thawing. Methods The ovaries of 1-day-old mice were collected and transplanted into the subrenal capsule of 8-12 week-old male F1 rats on the 2nd day (2d), 7th day (7d) and 14th day after transplantation 14d) Two groups of ovarian grafts were recovered. Light microscopy and electron microscopy of ovarian tissue before and after freezing and thawing and histological changes before and after transplantation, count the number of follicles. Results The survival rate of primordial follicles in mouse ovary after freezing and thawing was 87.9%. The ultrastructure of follicular cells and granulosa cells 7 days after ovarian transplantation were observed under light and electron microscope. The follicle structure returned to normal 14 days after transplantation. The 14th day after transplantation, the number of follicles and follicle survival rate (30.1%) in Salvia miltiorrhiza group were more than those in saline group (13.2%), the difference was significant (P <0.05). Conclusions Combined with light and electron microscopy, the effect of freeze-thaw process and transplantation on ovarian tissue can be evaluated more fully. The earlier stage of hypoxia and hypoxia in transplantation resulted in more serious damage to ovarian tissue than that in freezing and thawing. Danshen injection may promote the development of follicles in frozen-thawed mice after transplantation and reduce apoptosis.