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本文报导一种制备与提纯人的白细胞介素2(IL2)的方法。先将不同供者来源的外周血单核细胞混合,培养于10%胎牛血清RPMI1610培基中。37℃四天后,弃上清,再将细胞培养于1%胎牛血清RPMI1640培基中,同时加入多种B淋巴母细胞株(如DAUDI、IMK101等)以及纯质PHA,37℃培养18—24小时,收获上清液,即为粗制IL2。然后先后经50%及80%硫酸铵沉淀盐析、G—100凝胶过滤及BLUE-SEPHAROSE层析等步骤进行提纯,收集IL2活性最高的洗脱液即为纯化IL2。其活性比粗制IL2约高1,000倍。引言继发现植物血凝素可刺激人的细胞增殖后不久,又发现在这种细胞液中含有多种可
This article reports a method for the preparation and purification of human interleukin 2 (IL2). Peripheral blood mononuclear cells from different donor sources were first mixed and cultured in 10% fetal bovine serum RPMI1610 culture medium. After four days at 37 ° C, the supernatant was discarded and the cells were cultured in RPMI1640 medium supplemented with 1% fetal bovine serum. Meanwhile, various B lymphoblastoid cell lines (eg, DAUDI, IMK101, etc.) and pure PHA were added, 24 hours, harvested supernatant, namely crude IL2. Then purified by 50% and 80% ammonium sulfate precipitation salting-out, G-100 gel filtration and BLUE-SEPHAROSE chromatography. The highest IL2 activity was collected to purify IL2. It is about 1,000 times more active than crude IL2. Introduction Following the discovery of phytohemagglutinin can stimulate human cell proliferation shortly after, found in this cell solution contains a variety of