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大量单核细胞(MNC)及多核粒细胞(PMN)在关节滑液(SF)中浸润是类风湿关节炎(RA)的常见特征之一。浸润的细胞通过释放消化酶类及炎性细胞因子如IL-1,TNF-α等导致其病理改变。SF 中细胞浸润的过程是一被动过程,是由关节内活化的细胞所释放的趋化因子所致。许多因子如IL-1,TNF-α对中性粒细胞均显示趋化活性。但在体外,纯化的IL-1和TNF-α对中性粒细胞均无趋化活性。又发现一种新的由IL-1诱导单核细胞产生的中性粒细胞趋化因子(MDNCF),在体外显示直接趋化活性。这一因子现命名为IL-8。许多细胞如MNC、T 细胞、纤维母细胞等在炎性细胞因子如IL-1和TNF-α的刺激下均可产生IL-8。本文作者首次检测了RA 患者SF内是否显示IL-8活性及滑液细胞内IL-8mRNA 水平。结果10例RA 病人及9例非RA
Invasion of a large number of mononuclear cells (MNCs) and polymorphonuclear leukocytes (PMNs) in synovial fluid (SF) is one of the common features of rheumatoid arthritis (RA). Infiltrating cells cause their pathological changes by releasing digestive enzymes and inflammatory cytokines such as IL-1, TNF-α and so on. The process of cell infiltration in SF is a passive process induced by chemokines released by activated cells in the joints. Many factors such as IL-1 and TNF-α show chemotactic activity on neutrophils. However, purified IL-1 and TNF-α had no chemotactic activity on neutrophils in vitro. A new neutrophil chemokine (MDNCF), produced by IL-1-induced monocytes, was also found to show direct chemotactic activity in vitro. This factor is now named IL-8. Many cells such as MNC, T cells, fibroblasts and the like can produce IL-8 upon stimulation by inflammatory cytokines such as IL-1 and TNF-α. The authors first examined whether IL-8 activity and IL-8 mRNA levels in synovial cells were detected in SF patients. Results 10 RA patients and 9 non-RA patients