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目的:探讨转移相关基因2(metastasis-associated gene 2,MTA2)在食管癌组织中的表达以及对食管癌KYSE510细胞增殖和迁移的影响。方法:选取2014年在河北医科大学第四医院进行手术切除的50例食管癌组织及其相应的癌旁组织,应用实时荧光定量PCR检测MTA2和转录因子特异性蛋白1(specifi city protein 1,Sp1)m RNA的表达。将MTA2-si RNA和阴性对照si RNA片段转染至食管癌KYSE510细胞后,应用MTS实验和Transwell实验检测KYSE510细胞的增殖、迁移和侵袭情况,应用蛋白质印迹法检测KYSE510细胞中与上皮-间质转化(epithelialmesenchymal transition,EMT)相关蛋白的表达情况。结果:食管癌组织中MTA2 m RNA的表达水平显著高于相应的癌旁组织(P<0.05),并且与肿瘤的TNM分期(P<0.05)、淋巴结转移和血管浸润有关(P值均<0.01)。食管癌组织中Sp1 m RNA的表达水平显著高于相应的癌旁组织(P<0.05),且MTA2 m RNA的表达水平与Sp1 m RNA的表达水平呈正相关(r=0.407,P=0.028)。MTA2-si RNA转染组细胞的增殖、迁移和侵袭能力显著低于阴性对照组(KYSE510细胞转染阴性对照si RNA片段)和空白对照组(KYSE510细胞未进行任何转染)(P值均<0.05)。MTA2-si RNA转染组细胞中E-cadherin的表达水平上调(P<0.05),而N-cadherin、vimentin和CD24蛋白的表达水平下调(P<0.05、P<0.01和P<0.05)。结论:食管癌中MTA2 m RNA的表达水平上调,可能与转录因子Sp1的表达水平升高有关。MTA2-si RNA可抑制食管癌KYSE510细胞的增殖、迁移和侵袭,这一作用可能与EMT有关。
Objective: To investigate the expression of metastasis-associated gene 2 (MTA2) in esophageal carcinoma and its effect on the proliferation and migration of esophageal carcinoma KYSE510 cells. Methods: Fifty esophageal cancer tissues and corresponding paracancerous tissues resected in the Fourth Hospital of Hebei Medical University in 2014 were selected. Real-time fluorescent quantitative PCR was used to detect the expression of MTA2 and specifi c city1 ) m RNA expression. After transfection of MTA2-si RNA and negative control siRNA fragment into KYSE510 esophageal cancer cell line, MTS assay and Transwell assay were used to detect the proliferation, migration and invasion of KYSE510 cells. Western blotting was used to detect KYSE510 cells with epithelial-mesenchymal (Epithelialmesenchymal transition, EMT) related protein expression. Results: The expression of MTA2 m RNA in esophageal cancer tissues was significantly higher than that in the corresponding paracancerous tissues (P <0.05), and correlated with TNM stage (P <0.05), lymph node metastasis and vascular invasion (P <0.01) ). The expression of Sp1 m RNA in esophageal cancer tissues was significantly higher than that in corresponding adjacent tissues (P <0.05). The expression of MTA2 m RNA was positively correlated with the expression of Sp1 m RNA (r = 0.407, P = 0.028). The proliferation, migration and invasion ability of MTA2-si RNA transfected cells were significantly lower than that of negative control group (KYSE510 cells transfected with negative control si RNA fragment) and blank control group (KYSE510 cells without any transfection) (P < 0.05). The expression of E-cadherin was up-regulated in MTA2-si RNA transfected cells (P <0.05), while the expressions of N-cadherin, vimentin and CD24 were down-regulated (P <0.05, P <0.01 and P <0.05). Conclusion: The up-regulation of MTA2 mRNA expression in esophageal cancer may be related to the increased expression of transcription factor Sp1. MTA2-si RNA can inhibit the proliferation, migration and invasion of esophageal cancer KYSE510 cells, which may be related to EMT.