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目的:探讨原花青素(PC)对氨基脲(SEM)染毒小鼠肝脏损伤的影响。方法:50只成年昆明种(KM)小鼠,随机分为5组:溶剂对照组,SEM染毒组,SEM染毒+低、中、高剂量PC保护组;溶剂对照组,灌胃去离子水,每天1次,连续6周;SEM染毒组,前两周灌胃56.25 mg/(kg·bw)SEM溶液,后4周灌胃去离子水;低、中、高剂量PC保护组,前2周均灌胃56.25 mg/(kg·bw)SEM溶液,后4周分别灌胃100、200、400 mg/(kg·bw)PC溶液。末次灌胃24 h后,摘眼球采血后处死小鼠,测定相关检测指标。结果:实验6周后,氨基脲染毒组小鼠体重增长减慢,肝脏脏器系数降低,病理形态出现肝细胞变性坏死严重,肝索排列紊乱,胞浆疏松化和气球样变,肝功能血清中ALT、AST、GGT水平上升,SOD活性下降、MDA含量增高与溶剂对照组比较P<0.05;经PC尤其200、400 mg/(kg·bw)保护后,小鼠体重和肝脏脏器系数均增加,肝脏组织形态较SEM染毒组有所改善,肝功能ALT、AST、GGT水平趋向正常(下降),SOD活性升高,MDA含量降低与SEM染毒组相比,差异均有显著性(P<0.05)。结论:原花青素对氨基脲引起的小鼠肝脏损伤有保护作用;抗氧化作用是PC对SEM致小鼠肝脏损伤的保护机制之一。
Objective: To investigate the effect of proanthocyanidins (PC) on liver damage induced by semicarbazide (SEM) in mice. Methods: Fifty adult Kunming (KM) mice were randomly divided into five groups: solvent control group, SEM exposure group, SEM exposure group + low, medium and high dose PC protection group; solvent control group, Water, once a day for 6 weeks. The rats in SEM group were given 56.25 mg / (kg · bw) SEM solution twice a week for 2 weeks and then deionized water for 4 weeks. In the first two weeks, 56.25 mg / (kg · bw) SEM solution was given intragastrically, and 100,200,400 mg / (kg · bw) PC solution was given to the rats in the first two weeks. 24 h after the last gavage, the eyeballs were taken after sacrificing mice, the determination of the relevant test indicators. Results: After 6 weeks, the body weight of mice in semicarbazide group slowed down, the index of liver organ decreased, the pathological changes of hepatic cell degeneration and necrosis, disorder of hepatic cord, cytoplasm loosening and balloon-like change, liver function Serum ALT, AST, GGT levels increased, SOD activity decreased MDA content increased compared with the solvent control group P <0.05; PC, especially 200,400 mg / (kg · bw) protection, body weight and liver organ coefficient (P <0.05). Compared with SEM group, the morphology of liver tissue was improved, the levels of ALT, AST and GGT of liver function tended to be normal (decreased), the activity of SOD increased and the content of MDA decreased compared with that of SEM group (P <0.05). CONCLUSION: Proanthocyanidins protect mice liver caused by semicarbazide. Antioxidant effect is one of the protective mechanisms of PC against liver damage induced by SEM in mice.