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目的 通过重组人生长激素基因细胞移植为生长激素缺乏病 (ＧＨＤ)基因治疗的生物学表达研究奠定基础。方法 构建ｐＬＸＳＮｈＧＨ人生长激素 (ｈＧＨ)逆转录病毒表达载体后 ,脂质体转染包装细胞系ＰＡ317,提取包装细胞上清的病毒后 ,将病毒感染原代小鼠胚胎成纤维细胞 ,将细
OBJECTIVE: To lay the foundation for the study of biological expression of gene therapy for growth hormone deficiency (GHD) by recombinant human growth hormone gene cell transplantation. Methods After constructing pLXSNhGH human growth hormone (hGH) retroviral vector, the recombinant plasmid was transfected into PA317 cell line and then the virus was infected into primary mouse embryonic fibroblasts.