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目的 对 2 0 0 4年 4月起在江苏苏北及其邻近地区发生中、小学生爆发急性呼吸道疾病(ARD)流行的集中发热并伴有头痛、头晕、咽痛、扁桃体炎病例进行病因研究。方法 对流行区 2 0位患者的咽拭子标本分离病原体 ,并对阳性分离株进行免疫荧光、聚合酶链反应 (PCR)以及电镜检测。结果 MDCK(35代 )、LLC MK2、VeroE6、MRC 5 (39代 )分离培养均未出现细胞病变 ;军团菌培养亦未发现阳性结果 ;经人喉癌传代细胞 (Hep 2 )分离培养 ,2 0份标本中 13份出现了明显的细胞病变效应 ,电镜下可见细胞胞核内大量呈晶格状排列的典型腺病毒颗粒 ,直接免疫荧光试验及PCR检测均证实为腺病毒感染。结论 2 0 0 4年 4月发生在江苏省苏北地区中、小学急性呼吸道感染疫情的病原体为腺病毒。
Objective To study the etiology of fever, headache, dizziness, sore throat and tonsillitis in middle and primary school students in Jiangsu northern Jiangsu and its neighboring areas since April 2004. Methods The pathogens were isolated from throat swab specimens of 20 patients in epidemic area and immunofluorescence, polymerase chain reaction (PCR) and electron microscopy were performed on the positive isolates. Results No cytopathic effect was found in MDCK (35 passages), LLC MK2, VeroE6 and MRC 5 (passage 39). No positive results were found in Legionella cultures. A significant cytopathic effect was observed in 13 of the samples. A large number of typical adenovirus particles arranged in a lattice in the nucleus of cells were observed under electron microscope. Both adenovirus and adenovirus were detected by direct immunofluorescence assay and PCR assay. Conclusion In April 2004, the pathogen of acute respiratory infection in primary and secondary schools in northern Jiangsu Province was adenovirus.