目的　筛选和克隆卵巢上皮癌相关基因。　方法　用 3种锚定引物和 8种随机引物 ,共 2 4种组合进行PCR扩增 ,通过 DD- RTPCR分析正常卵巢上皮和卵巢上皮癌 m RNA的基因表达差异 ,将差异片段分离出来 ,并回收 ,克隆和进行斑点杂交鉴定、DNA序列测定 ,并通过国际互联网络检索 Gen Bank进行同源性分析。　结果　获得30个差异表达的片段 ,经斑点杂交初步筛选 ,克隆 11个差异显示的阳性片段。选取其中的 5个进行序列分析。　结论　计算机同源性分析表明 ,5个均为新基因片段 ,已收录入 Gen Bank。 Objective To screen and clone ovarian epithelial cancer related genes. METHODS: Three kinds of anchor primers and 8 random primers were used for PCR amplification in 24 combinations. The gene expression differences of m RNA in normal ovarian epithelial and epithelial ovarian cancers were analyzed by DD-RT PCR, and the differential fragments were isolated and recovered. , Cloning and dot blot hybridization identification, DNA sequence determination, and GenBank analysis via the Internet for homology analysis. Results Thirty differentially expressed fragments were obtained and screened by dot blot to clone 11 positively expressed fragments. Select 5 of them for sequence analysis. Conclusion Computer homology analysis showed that 5 genes are new gene fragments and have been included in Gen Bank.