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为了确定AML是一个还是多个造血细胞系受累,作者设计了一种技术:同时确定一种细胞是否为恶性的,以及它隶属的细胞系。用数值表示的克隆异常染色体作为肿瘤细胞标记。例如:三体8和单体7在AML是肿瘤的明确标记;用碱性磷酸酶抗碱性磷酸酶染色的单克隆抗体识别粒单或红、或巨核细胞系。用来确定粒单系的McAb有My_7、PM_(81)、LeuMI、My_4和OKM_5,用鼠的抗葡萄球菌蛋白A标记红细胞系,用Y_2/_(51)和因子Ⅷ确定巨核细胞系。一种抗人γ-干扰素的IgG_1McAb用作阴性对照。检查了12例AML的骨髓分裂中期细胞。8例
To determine whether AML is involved in one or more hematopoietic cell lines, the authors devised a technique that simultaneously determines whether a cell is malignant or not, as well as the cell line to which it belongs. Numeric clonally abnormal chromosomes are used as tumor cell markers. For example, trisomy 8 and monomer 7 are well-defined markers of tumor in AML; monoclonal or red, or megakaryocyte lines are identified by alkaline phosphatase-resistant alkaline phosphatase-stained monoclonal antibodies. The McAbs used to identify the single line were identified as My_7, PM_ (81), LeuMI, My_4 and OKM_5. The red blood cell lines were stained with anti-staphylococcal protein A and the megakaryocytic cell lines were identified with Y_2 / _ (51) and factor VIII. An anti-human gamma-interferon IgG1McAb was used as a negative control. 12 cases of metaphase cells of AML were examined. 8 cases