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目的:观察10种黄酮类化合物对糖尿病致病机制中4条重要通路的多靶点抑制作用,发挥中药多靶点、综合治疗的优势。方法:从正常大鼠脑组织中提取半纯化的蛋白激酶C(PKC)、肾脏组织中提取醛糖还原酶(AR)粗酶,Fenton试剂体外激发正常大鼠脑组织脂质过氧化生成丙二醛(MDA)及体外合成高级糖化终产物(AGEs),将上述酶或合成物与10种黄酮类化合物(终浓度为1 mg.mL-1)作用,观察黄酮类化合物对上述酶或合成物的抑制作用。结果:黄芩苷和灯盏花素对AR,PKC,AGEs及MDA均有不同程度的抑制作用。结论:上述结果提示此两种黄酮类化合物可以通过抑制上述4条通路来预防糖尿病及其并发症的发生,为进一步的机制研究及临床应用提供良好的实验依据。
OBJECTIVE: To observe the multi-target inhibitory effect of 10 flavonoids on four important pathways in the pathogenic mechanism of diabetes, and to make full use of the advantages of multi-targets and comprehensive treatment of traditional Chinese medicine. Methods: Semi-purified protein kinase C (PKC) was extracted from the brain tissue of normal rats. The crude enzyme of aldose reductase (AR) was extracted from the kidneys. Fenton reagent stimulated the lipid peroxidation of normal rat brain to generate propanediol Aldehyde (MDA) and in vitro synthesis of advanced glycation end products (AGEs), the above enzyme or composition with 10 flavonoids (final concentration of 1 mg.mL-1) role observed flavonoids on the above enzyme or composition Inhibition. Results: Baicalin and breviscapine had different inhibitory effects on AR, PKC, AGEs and MDA. Conclusion: The above results suggest that the two flavonoids can prevent the occurrence of diabetes and its complications by inhibiting the above four pathways, and provide a good experimental basis for further mechanism research and clinical application.