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许多细胞可通过PKC 跨膜信号的传递而产生某些生物活性介质。LPS 可促使人单核细胞PKC 从胞浆移位到胞膜,从而诱导细胞产生和分泌IL-1。采用游离LPS,MLV-LPS(多层磷脂泡LPS),Lyo-MLV-LPS(水溶性脂质体表面LPS 经用LPS 重悬后制成)和LPS-脂质体四种不同形式的LPS 以及H7和星状孢子两种PKC 抑制剂,通过IL-1生物学活性方法检测单核细胞IL-1产生水平,应用免疫斑点法和[~3H]PBDu 结合法检测胞质中、胞膜中PKC 分布,研究了PKC在单核细胞IL-1诱导产生过程中的作用。结果表明:(1)游离LPS 或Lyo-MLV-LPS 促使IL-1α、β的产生、分泌及PKC
Many cells produce certain biologically active mediators through the transmission of PKC transmembrane signals. LPS can promote human monocyte PKC translocation from the cytoplasm to the membrane, thereby inducing cells to produce and secrete IL-1. LPS-LPS (LPS), Lyo-MLV-LPS (water-soluble liposome surface LPS after resuspended with LPS) and LPS-liposomes in four different forms of LPS and H7 and astrovirus were used to detect the level of IL-1 in monocytes by using IL-1 biological activity method. Immunocytochemistry and [~ 3H] PBDu binding assay were used to detect the levels of PKC Distribution, the role of PKC in monocyte IL-1 induction was investigated. The results showed that: (1) Free LPS or Lyo-MLV-LPS promoted the production and secretion of IL-1α, β and PKC