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炮制方法:参照传统的炮制方法将吴茱萸加30倍热水(70℃)缓缓搅拌15min,滤过,依此方法反复操作7次,将各炮制阶段得到的吴茱萸冷冻干燥后备用。样品液的制备方法:称取吴茱萸粉末100mg,加100ml甲醇回流提取30min,提取液滤过后,置旋转蒸发器蒸发。残留物溶解在1Oml甲醇中,取10μ1供HPLC分析。生物碱的含量变化:炮制前吴茱萸中羟基吴茱萸碱(1)为0.98％、吴茱萸碱(Ⅱ)为1.13％,吴茱萸次碱(Ⅲ)为1.34％,吴茱萸新碱evoearpine(Ⅳ)为1.65％。炮制后其含
Processing method: According to the traditional method of concoction, add 30 times hot water (70°C) to Wujing to stir slowly for 15min, filter it, and repeat the operation 7 times according to this method. Sample liquid preparation method: Weigh 100 mg of Evodia rutaecarpa powder and add 100 ml of methanol to reflux for 30 min. After the filtration of the extract, we place a rotary evaporator to evaporate. The residue was dissolved in 10 ml of methanol and 10 μl was taken for HPLC analysis. Changes in alkaloid content: Before processing, the content of evodiamine (1) in Evodia rutaecarpa (1) was 0.98%, that of evodiamine (II) was 1.13%, that of rutacarpine (III) was 1.34%, and that of evodiamine evorepine (IV) was 1.65%. After processing it contains