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采用线栓法MCAO缺血动物模型 ,观察了不同时程电针对局灶性脑缺血大鼠脑梗死、脑肿胀、纹状体 ,皮层神经元数量的影响 ,同时观察了缺血再灌注和电针对碱性成纤维生长因子 (bFGF)免疫活性在纹状体梗死周边区和额顶叶皮质表达的影响。结果表明 :在中动脉阻塞引起的脑缺血后 ,可见受损脑侧有明显的脑梗死 ,脑肿胀 ,神经元丢失 ,并伴随有显著的bFGF免疫活性物质表达增加。在纹状体梗死周边区 ,bFGF免疫活性物质主要位于星型胶质细胞 ,但在紧临梗塞的区域 ,一些神经元中也有bFGF表达。在额顶叶皮质 ,神经元中有非常显著的bFGF免疫活性增加。对于减少脑梗塞和脑肿胀 ,无论是缺血或再灌期间电针均能取得显著性效果 ,并且 ,对于减少脑肿胀 ,再灌期间电针作用可能较强。缺血再灌后 ,在纹状体梗死周边区和额顶叶皮质bFGF免疫活性表达增加。缺血或再灌期间电针均能进一步上调bFGF免疫活性表达。结果提示 ,在纹状体 ,胶质细胞可能通过表达bFGF起到神经保护作用 ;在额顶叶皮质 ,神经元可能主要通过分泌bFGF而实现自我保护。电针的神经保护效应可能部分通过增强内源性bFGF表达实现。
The effect of electroacupuncture at different time points on the cerebral infarction, brain swelling, striatum and cortical neurons in focal cerebral ischemia rats was observed by using MCAO model. The effects of ischemia / reperfusion Effects of Electroacupuncture on the Basic Fibroblast Growth Factor (bFGF) Immunoreactivity in the Peripheral Area and the Frontal Lobes of the Striatum Infarction. The results showed that after cerebral ischemia caused by occlusion of the middle artery, obvious cerebral infarction, brain swelling and neuronal loss were observed in the injured brain, accompanied by significant increase in the expression of bFGF immunoreactive substance. In the peripheral region of the striatum, the bFGF immunoreactive substance is predominantly located on astrocytes, but bFGF is also expressed in some neurons in the immediate infarct region. In the frontal cortex, neurons have a significant increase in bFGF immunocompetence. Electro-acupuncture can achieve significant effects both for cerebral infarction and brain swelling during either ischemia or reperfusion, and electro-acupuncture may be more effective for reducing brain swelling during reperfusion. After ischemia / reperfusion, the expression of bFGF immunoreactivity increased in the peripheral area of the striatum and in the frontal cortex. Electroacupuncture can further up-regulate bFGF immunoreactivity during ischemia or reperfusion. The results suggest that in the striatum, glial cells may play a neuroprotective role through the expression of bFGF; in the frontal cortex, neurons may self-protect mainly through the secretion of bFGF. The neuroprotective effect of electroacupuncture may be partially achieved by enhancing the expression of endogenous bFGF.