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采用正交设计，获得了苏云金芽胞杆菌菌株９４（Ｂｔ９４）的最适液体培养基配方：葡萄糖质量浓度为０．７６ｋｇＬ－１、胰蛋白胨＋酵母浸膏为０．８ｋｇＬ－１、无机盐为０．１ｋｇＬ－１．采用该培养基进行摇床培养，活芽孢数可达１０１×１０１１个Ｌ－１．用摇瓶培养液对斜纹夜蛾和小菜蛾进行毒力测定，其ＬＣ５０分别为３５５．９９５１±２．６０９８和５８．２９０８±１．４２６２ｍＬＬ
Orthogonal design was used to obtain the optimum liquid medium formula of Bacillus thuringiensis strain 94 (Bt94): glucose concentration 0.76kgL-1, tryptone + yeast extract 0.8kgL-1, inorganic salt 0 .1kgL-1. Using this medium for shaker culture, the number of viable spores can reach 101 × 1011 L-1. The virulence of Spodoptera litura and Plutella xylostella was determined by shake flask culture medium with LC50 of 355.9951 ± 2.6098 and 58.2908 ± 1.4262mLL