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目的研究不同强度的短期间歇性减压低氧(IHH)对大鼠血液及心血管组织中抗氧化物质的影响。方法将30只6周龄雄性SD大鼠分为对照组(n=10)、IHH 3 500 m组(n=10)和IHH 5 000 m组(n=10)。IHH 3 500 m组和IHH 5 000 m组动物置于低压氧舱分别接受相当3 500 m和5 000 m海拔高度的减压低氧处理,每天4 h,共持续8 d。对照组动物除不接受低氧处理外,其他条件与低氧动物相同。低氧实验结束后,测定血浆、心肌及主动脉组织中的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性与丙二醛(MDA)含量,并测定血浆中尿酸含量。结果与对照组相比,IHH 5 000 m组大鼠血浆CuZn-SOD活性增强,Mn-SOD活性降低,心肌总SOD及Mn-SOD活性均增强,血浆和心肌中的CAT活性增强,MDA含量增加,血浆尿酸含量增加(P<0.05);但IHH 5 000 m组大鼠的主动脉SOD、CAT、MDA与对照组相比,差异均无统计学意义(P>0.05)。与对照组相比,IHH3 500 m组大鼠血浆Mn-SOD活性增强,差异有统计学意义(P<0.05);但血浆尿酸、心肌与主动脉的CAT、MDA差异均无统计学意义(P>0.05)。结论中等强度的短期IHH可诱导血液及心肌组织抗氧化物质升高。
Objective To study the effects of different intensity short-term intermittent hypobaric hypoxia (IHH) on antioxidants in blood and cardiovascular tissue of rats. Methods Thirty 6-week-old male Sprague Dawley rats were divided into control group (n = 10), IHH 3 500 m group (n = 10) and IHH 5000 m group (n = 10). IHH 3 500 m group and IHH 5 000 m group were placed in the hyperbaric chamber for 3 h and 3 000 m, respectively, and subjected to hypoxia and hypoxia for 3 h and 4 h, respectively, for 8 days. The animals in the control group were the same as the hypoxic animals except that they did not receive hypoxia. After hypoxia, SOD, catalase (CAT) and malondialdehyde (MDA) contents were measured in plasma, myocardium and aorta, and the contents of uric acid in plasma were determined. Results Compared with the control group, the activity of CuZn-SOD and the activity of Mn-SOD in plasma of the IHH 5000 m group were significantly increased, the activity of total SOD and Mn-SOD increased, the activity of CAT in the plasma and myocardium increased, and the content of MDA increased (P <0.05). However, the SOD, CAT, MDA in the aorta of IHH 5000 m group were not significantly different from the control group (P> 0.05). Compared with the control group, plasma Mn-SOD activity in IHH3 500 m group increased significantly (P <0.05), but there was no significant difference in plasma uric acid, CAT, MDA in myocardium and aorta (P > 0.05). CONCLUSIONS: Moderate-intensity short-term IHH induces an increase in anti-oxidant substances in blood and myocardium.