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目的:观察大黄虫丸抗球囊损伤内皮诱导的家兔动脉粥样硬化的作用机制。方法:采用机械损伤动脉内皮辅以高脂饲料喂养的方法建立家兔动脉粥样硬化模型。将雄性新西兰家兔分为6组:正常对照组、假手术组、模型组、阳性对照组及大黄虫丸低、高剂量组。正常对照组、假手术组与模型组给予蒸馏水,阳性对照组给予丹参0.5 g/kg,大黄虫丸低、高剂量组分别给予大黄虫丸0.9、1.8 g/kg。灌胃给药,1次/d,连续60 d。实验结束时,用分光光度法测定血清丙二醛(MDA)、超氧化物歧化酶(SOD)、一氧化氮(NO)及血管壁髓过氧化物酶(MPO),SP免疫组织化学法测定血管平滑肌增殖细胞核抗原(PCNA)和BCL-2的表达。结果:大黄虫丸能降低血清MDA水平和血管壁MPO活性,升高SOD活性和NO含量,减少血管壁PCNA和BCL-2的表达。结论:大黄虫丸通过抗脂质过氧化作用、保护血管内皮、抑制VSMCs增殖和促进VSMCs凋亡等机制,抑制球囊损伤内皮诱导的动脉粥样硬化形成和发展。
Objective: To observe the mechanism of Dahuang Zhechong pill on the atherosclerosis induced by endothelium induced by balloon injury in rabbits. Methods: Rabbit models of atherosclerosis were established by means of mechanical injury of endothelium - derived artery supplemented with high fat diet. Male New Zealand rabbits were divided into 6 groups: normal control group, sham operation group, model group, positive control group and rhubarb worm pill low and high dose group. The normal control group, sham operation group and model group were given distilled water. The positive control group was given Salvia Miltiorrhiza 0.5g / kg, rhubarb Zhechong pill low, high dose group were given rhubarb Zhechong pill 0.9,1.8 g / kg. Gavage, 1 / d, continuous 60 d. At the end of the experiment, the levels of malondialdehyde (MDA), superoxide dismutase (SOD), nitric oxide (NO) and vascular wall myeloperoxidase (MPO) were determined by spectrophotometry and SP immunohistochemistry Vascular Smooth Muscle Proliferating Cell Nuclear Antigen (PCNA) and BCL-2 Expression. Results: Rhubarb Zhechong Pill can reduce serum MDA and MPO activity, increase SOD activity and NO content, and decrease the expression of PCNA and BCL-2 in the vascular wall. Conclusion: Dahuang Zhechong pill inhibits the formation and development of atherosclerosis induced by balloon injury through anti-lipid peroxidation, protection of vascular endothelium, inhibition of VSMCs proliferation and apoptosis of VSMCs.