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目的:观察Snail mRNA与E-cad-herin mRNA在喉鳞癌组织中的表达及其与临床病理特征的关系。方法:应用原位分子杂交方法,分别检测Snail mRNA与E-cadherin mRNA在60例喉鳞癌、30例不典型增生和20例慢性炎症组织中的表达。结果:Snail mRNA在喉鳞癌中的表达高于不典型增生和慢性炎症组织中的表达,而E-cadherin mRNA在喉鳞癌中的表达低于不典型增生和慢性炎症组织中的表达,差异均有统计学意义,P值均<0.05;Snail mRNA、E-cadherin mRNA与喉鳞癌甲状软骨累及淋巴结转移、TNM分期、T分期、临床分型、病理分级有关(P值均<0.05),而两者均与性别、年龄、肿瘤大小无关,P值均>0.05;喉鳞癌Snail mRNA和E-cadherin mRNA的表达呈明显负相关,r=-0.504,P<0.05。结论:E-cadherin mRNA低表达与Snail mRNA高表达可能是喉黏膜恶性转变以及喉鳞癌发生浸润转移的重要生物学标志,联合检测E-cadherin mRNA和Snail mRNA对预测喉鳞癌浸润转移有重要意义。
Objective: To observe the expression of Snail mRNA and E-cad-herin mRNA in laryngeal squamous cell carcinoma and its relationship with clinicopathological features. Methods: The expressions of Snail mRNA and E-cadherin mRNA in 60 cases of laryngeal squamous cell carcinoma, 30 cases of dysplasia and 20 cases of chronic inflammation were detected by in situ hybridization. Results: The expression of Snail mRNA in laryngeal squamous cell carcinoma was higher than that in atypical hyperplasia and chronic inflammation, while the expression of E-cadherin mRNA in laryngeal squamous cell carcinoma was lower than that in atypical hyperplasia and chronic inflammation (All P values <0.05). The expressions of Snail mRNA and E-cadherin mRNA were correlated with lymph node metastasis of thyroid cartilage in laryngeal squamous cell carcinoma, TNM stage, T stage, clinical classification and pathological grade P <0.05). The expressions of Snail mRNA and E-cadherin mRNA in laryngeal squamous cell carcinoma were negatively correlated (r = -0.504, P <0.05), both of which were unrelated to gender, age and tumor size. Conclusion: The low expression of E-cadherin mRNA and the high expression of Snail mRNA may be important biomarkers of malignant transformation of the laryngeal squamous cell carcinoma and invasion and metastasis of laryngeal squamous cell carcinoma. The combined detection of E-cadherin mRNA and Snail mRNA is important for predicting the invasion and metastasis of laryngeal squamous cell carcinoma significance.