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目的探讨子痫前期患者胎盘组织及血清中信号转导和转录激活因子4(STAT4)以及信号转导和转录激活因子6(STAT6)的表达及意义。方法 1采用Western Blot法及酶连免疫吸附试验(ELASIA)法检测30例正常晚期妊娠孕妇(对照组)和50例子痫前期患者(子痫前期组,其中轻度22例、重度28例)胎盘组织和血清中STAT4、STAT6的蛋白表达。结果 STAT4在正常妊娠组、子痫前期轻度组与重度组胎盘和血清组织中的含量随着病情的加重呈增加趋势,轻、重度子痫前期组胎盘和血清组织中STAT4的表达均高于对照组(P<0.01)。STAT6在正常妊娠组、子痫前期轻度组与重度组的胎盘及血清含量随着病情的加重呈减低的趋势,轻、重度子痫前期组胎盘和血清组织中STAT6的表达均低于对照组(P<0.01)、其中重度组STAT6表达低于轻度组(P<0.05)。子痫前期组和正常组胎盘和血清组织中STAT4与STAT6的表达水平均呈负相关。结论 STAT4、STAT6共同参与子痫前期的病理生理改变,并与病情严重症程度有一定关系。子痫前期胎盘、血清中STAT4的高表达和STAT6的低表达可能诱导T辅助淋巴细胞1/2失衡,进而导致子痫前期的发病。
Objective To investigate the expression and significance of signal transducers and activators of transcription 4 (STAT4), signal transducer and activator of transcription 6 (STAT6) in placenta and serum of patients with preeclampsia. Method 1 Western blot and enzyme-linked immunosorbent assay (ELASIA) were used to detect the expression of placenta in 30 normal pregnant women (control group) and 50 preeclampsia patients (preeclampsia group, mild 22 cases, severe 28 cases) Tissue and serum STAT4, STAT6 protein expression. Results The levels of STAT4 in placenta and serum of mild and severe preeclampsia group increased with the severity of the disease. The expression of STAT4 in placenta and serum of mild and severe preeclampsia group was higher than that of STAT4 Control group (P <0.01). STAT6 in the normal pregnancy group, mild and severe preeclampsia placenta and serum levels of the group with the severity of the disease showed a downward trend, mild and severe preeclampsia placenta and serum tissue STAT6 expression were lower than the control group (P <0.01). The expression of STAT6 in severe group was lower than that in mild group (P <0.05). The expression levels of STAT4 and STAT6 in placenta and serum of preeclampsia group and normal group were negatively correlated. Conclusion STAT4 and STAT6 are involved in the pathophysiological changes of preeclampsia and have a certain relationship with the severity of the disease. In preeclampsia, high expression of STAT4 and low expression of STAT6 in the placenta and serum may induce 1/2 imbalance of T helper lymphocytes, leading to the development of preeclampsia.