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目的探讨纳米微粒介导耐药基因mdr-1、mrp反义寡脱氧核苷酸(antisense oligodeoxynucleotide,ASODN)转染对多药耐药乳腺癌MCF-7/ADR细胞的影响。方法采用纳米微粒介导mdr-1、mrp-ASODN转染耐药细胞株MCF-7/ADR,RT-PCR、Western blot检测转染48h后细胞耐药基因mdr-1、mrp的表达;MTT法检测经转染后MCF-7/ADR细胞对阿霉素(ADR)、表柔比星(epirubicin)、5-氟脲嘧啶(5-FU)和紫杉醇(paclitaxel)的敏感性。结果纳米微粒介导mdr-1、mrpASODN转染48h后,MCF-7/ADR细胞的mdr-1、mrp在mRNA和蛋白质表达水平上均显著降低(P<0.05);细胞对ADR、表柔比星、5-氟脲嘧啶和紫杉醇的耐药指数均显著降低(P<0.05)。结论耐药基因反义寡脱氧核苷酸能在体外抑制耐药基因的表达,从而提高细胞的药物敏感性;纳米微粒具有较好的体外介导反义寡脱氧核苷酸转染效果。
Objective To investigate the effect of multidrug-resistant breast cancer MCF-7 / ADR cells transfected with antisense oligodeoxynucleotide (mdr-1) and mrp-mediated antisense oligodeoxynucleotide (ASODN) mediated by nanoparticles. Methods The mdr-1 and mrp-ASODN were transfected into MCF-7 / ADR cells by MTT assay. The expression of mdr-1 and mrp genes were detected by RT-PCR and Western blot 48 h after transfection. The sensitivity of transfected MCF-7 / ADR cells to doxorubicin (ADR), epirubicin, 5-fluorouracil (5-FU) and paclitaxel was examined. Results After transfected with mdr-1 and mrpASODN, the mRNA and protein expression levels of mdr-1 and mrp in MCF-7 / ADR cells decreased significantly (P <0.05) Star, 5 - fluorouracil and paclitaxel were significantly lower resistance index (P <0.05). Conclusion Antisense oligodeoxynucleotides of drug-resistant gene can inhibit the expression of drug-resistance genes in vitro and thus enhance the drug sensitivity of cells. Nanoparticles have better in vitro antisense oligodeoxynucleotide transfection effect.