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目的：为研究日本血吸虫（中国大陆株）22．6kDa抗原的免疫保护性。方法：将其编码cDNA经PCR扩增后亚克隆入载体质粒PGEX—1λt进行表达，并用重组抗原免疫了一批昆明鼠。结果：与对照组相比，重组抗原免疫小鼠获得了38．93％的减虫率。结论：证明重组的日本血吸虫22．6kDa抗原可诱导宿主抗血吸虫感染的部分保护力。
Objective: To study the immunoprotection of 22.6kDa antigen of Schistosoma japonicum (Chinese mainland strain). Methods: The cDNA encoding it was subcloned into vector pGEX-1λt after PCR amplification, and a group of Kunming mice were immunized with the recombinant antigen. Results: Compared with the control group, mice immunized with recombinant antigen achieved a worm reduction rate of 38.93%. CONCLUSIONS: It was demonstrated that the recombinant Schistosoma japonicum 22.6 kDa antigen can induce partial protection of the host against infection by schistosomiasis.