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目的:建立甜叶菊的检查、定性鉴别及含量测定方法,为其质量控制提供科学依据。方法:按照中国药典委员会对中药质量标准的技术要求,对甜叶菊进行了性状、显微及薄层色谱鉴别,并对杂质、水分、总灰分、醇浸出物及指标成分(甜菊苷及莱鲍迪苷A)含量进行测定。结果:性状、显微鉴别、薄层色谱鉴别及含量测定方法重复性好,专属性强;根据24批甜叶菊分析结果,拟定甜叶菊杂质不得过4%,水分不得过11%,总灰分不得过9%,醇浸出物不得低于41%,甜菊苷含量不得低于2%,莱鲍迪苷A含量不得低于3%。结论:建立的检查、定性鉴别及含量测定方法,能有效控制甜叶菊的质量。
Objective: To establish the method of inspection, qualitative identification and content determination of stevia so as to provide a scientific basis for its quality control. Methods: According to the technical requirements of the Chinese Pharmacopoeia Commission on the quality standard of Chinese medicine, traits of Stevia were identified by microscopic and TLC, and the contents of impurity, water, total ash, alcohol extract and index components Diidin A) content was measured. Results: Traits, microscopic identification, TLC identification and content determination methods were reproducible and specific. According to 24 batches of stevia analysis, no more than 4% of stevia was allowed, the water content should not exceed 11%, and the total ash content was not Over 9% alcohol extract not less than 41%, stevioside content of not less than 2%, rebaudioside A content of not less than 3%. Conclusion: The establishment of the inspection, qualitative identification and determination of content, can effectively control the quality of stevia.