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目的:观察体内外实验中受试者饮用啤酒后各种血清酶活性的变化情况。设计:观察对比实验。单位:泰山医学院基础医学研究所。对象:实验于2005-03/04在泰山医学院基础医学研究所完成。选择泰山医学院学生17名,年龄19～35岁,包括本科生和研究生,实验前均签署同意书。方法:①体内实验:受试者统一正常饮食后3h采静脉血3mL,作为对照。然后立即口服啤酒4mL/kg,分别于15,30,45,60,90,120,180min后各采血3mL,测定血液中丙氨酸氨基转移酶、天冬氨酸氨基转移酶、γ-谷氨酰转肽酶、碱性磷酸酶、肌酸激酶、乳酸脱氢酶、淀粉酶、脂肪酶活性的变化。②体外实验:选取17份新鲜受试者血清,分别加入两个试管中,每管0.5mL。对照管加入20μL生理盐水;测定管中加入20μL啤酒溶液,观察啤酒对以上各种酶活性的直接影响。主要观察指标:体内外实验中血清丙氨酸氨基转移酶、天冬氨酸氨基转移酶、γ-谷氨酰转肽酶、碱性磷酸酶、肌酸激酶、乳酸脱氢酶、淀粉酶及脂肪酶的活性。结果:纳入17名学生,全部进入结果分析,无脱落。①体内实验:啤酒可显著降低血清天冬氨酸氨基转移酶活性(418.08±58.68,383.41±63.01)nkat/L,显著升高血清碱性磷酸酶活性(3678.57±436.25,3962.96±400.91)nkat/L(χ2=19.00～20.00,P<0.01),其余酶活性均有不同程度的升高。②体外实验:啤酒在体外对各种酶活性均有一定程度的抑制作用。结论:啤酒在体内外对酶活性均有一定影响,从而影响机体代谢,过量饮用会影响健康。在常规血清酶学检测中,应避免患者饮用啤酒所造成的干扰,以确保实验结果的准确可靠。
OBJECTIVE: To observe the changes of serum enzyme activities after drinking beer in vivo and in vitro. Design: observe the contrast experiment. Unit: Institute of Basic Medical Sciences, Taishan Medical College. Subject: The experiment was performed at the Institute of Basic Medical Sciences, Taishan Medical College from March to April 2005. Seventeen Taishan Medical College students aged 19-35 years were enrolled, including undergraduates and graduate students, both of whom signed a consent form before the experiment. Methods: ① In vivo experiments: 3 mL of venous blood was collected 3 h after the normal diet of the subjects as a control. Immediately after that, 4 mL / kg of beer was orally administered, and 3 mL of each blood was taken after 15, 30, 45, 60, 90, 120 and 180 minutes respectively for determination of alanine aminotransferase, aspartate aminotransferase, γ- Changes in enzyme, alkaline phosphatase, creatine kinase, lactate dehydrogenase, amylase, lipase activity. ② In vitro experiments: 17 fresh subjects were selected serum, were added to two test tubes, each tube 0.5mL. Control tube was added 20μL saline; determination of the tube by adding 20μL beer solution to observe the direct impact of beer on the various enzyme activity. MAIN OUTCOME MEASURES: Serum alanine aminotransferase, aspartate aminotransferase, γ-glutamyl transpeptidase, alkaline phosphatase, creatine kinase, lactate dehydrogenase, amylase, Lipase activity. Results: 17 students were enrolled in the analysis of the results, without loss. (1) In vivo experiments: Beer significantly reduced nkat / L aspartate aminotransferase activity (418.08 ± 58.68, 383.41 ± 63.01) L (χ2 = 19.00 ~ 20.00, P <0.01), and the activity of other enzymes increased to some extent. ② in vitro experiments: beer in vitro activity of various enzymes have a certain degree of inhibition. Conclusion: Beer has a certain impact on enzyme activity both in vitro and in vivo, thus affecting the body metabolism, excessive drinking will affect the health. In routine serum enzyme tests, patients should avoid drinking beer caused by interference to ensure that the experimental results are accurate and reliable.