[Objective]To explore the anti-inflammatory and analgesic effects of 12 Guangdong Native Heat-clearing Herbs with identical dose( 16 g/kg) and to provide reliable experiment basis for the clinical development and application of anti-inflammatory drugs. [Methods]To explore the anti-inflammatory and analgesic effects of 12 Guangdong Native Heat-clearing Herbs by acetic acid writhing,hot plate method,ear swelling caused by xylene in mice and celiac capillary permeation in mice induced by acetic acid. [Results]After intragastric administration of drugs( 16 g / kg) in mice for 7 days,the inhibiting effects of Radix Helicteris,Thinleaf Adina Fruit,Nervilia fordii,Radix Ilicis Asprellae and Trikeraia hookeri on ear swelling caused by xylene in mice were significant and the inhibiting effects of which on the increase of capillary permeability in mice induced by acetic acid were also significant( P < 0. 05). Radix Eupatorii Chinensis,Pronephrium triphyllum and Bidens biternata could inhibit ear swelling caused by xylene in mice significantly and Evodia lepta Merr,ovate leaf Holly Bark and Rubus parvifolius could inhibit the increase of capillary permeability in mice significantly( P < 0. 05). The effects of 12 Guangdong Native Heat-clearing Herbs on thymus and spleen were not significant( P > 0. 05); Trikeraia hookeri,Radix Ilicis Asprellae,ovate leaf Holly Bark,Radix Helicteris, Nervilia fordii and Herba Polygoni Chinensis could evidently reduce the times of writhing response in mice induced by acetic acid and increase the threshold of pain in mice induced by thermal stimulus; Evodia lepta Merr and Pronephrium triphyllum could evidently reduce the times of writhing response in mice induced by acetic acid,ovate leaf Holly Bark could significantly prolong the latent time of writhing response in mice induced by acetic acid( P < 0. 05) and Bidens biternata could significantly increase the threshold of pain in mice induced by thermal stimulus. [Conclusions]Radix Helicteris,Thinleaf Adina Fruit,Nervilia fordii,Herba Polygoni Chinensis,Radix Ilicis Asprellae,Trikeraia hookeri, Radix Eupatorii Chinensis,Pronephrium triphyllum,Bidens biternata,Evodia lepta Merr,ovate leaf Holly Bark and Rubus parvifolius had anti-inflammatory action; Radix Helicteris,Nervilia fordii,Herba Polygoni Chinensis,Radix Ilicis Asprellae,Trikeraia hookeri,Pronephrium triphyllum,Bidens biternata,Evodia lepta Merr and ovate leaf Holly Bark,etc. had analgesic effect. [Objective] To explore the anti-inflammatory and analgesic effects of 12 Guangdong Native Heat-clearing Herbs with identical dose (16 g / kg) and to provide reliable experiment basis for the clinical development and application of anti-inflammatory drugs. [Methods] To explore the anti-inflammatory and analgesic effects of 12 Guangdong Native Heat-clearing Herbs by acetic acid writhing, hot plate method, ear swelling caused by xylene in mice and Celiac capillary permeation in mice induced by acetic acid. [Results] After intragastric administration of drugs (16 g / kg) in mice for 7 days, the inhibiting effects of Radix Helicteris, Thinleaf Adina Fruit, Nervilia fordii, Radix Ilicis Asprellae and Trikeraia hookeri on ear swelling caused by xylene in mice were significant and the inhibiting effects of which on the increase of capillary permeability in mice induced by acetic acid were also significant (P <0.05). Radix Eupatorii Chinensis, Pronephrium triphyllum and Bidens biternata could inhibit ear swelling caused by xylene in mice significantly and Evodia lepta Merr, ovate leaf Holly Bark and Rubus parvifolius could inhibit the increase of capillary permeability in mice significantly (P <0.05). The effects of 12 Guangdong Native Heat-clearing Herbs on thymus and Spleen were not significant (P> 0.05); Trikeraia hookeri, Radix Ilicis Asprellae, ovate leaf Holly Bark, Radix Helicteris, Nervilia fordii and Herba Polygoni Chinensis could evidently reduce the times of writhing response in mice induced by acetic acid and increase the threshold of pain in mice induced by thermal stimulus; Evodia lepta Merr and Pronephrium triphyllum could evidently reduce the times of writhing response in mice induced by acetic acid, ovate leaf Holly Bark could significantly prolong the latent time of writhing response in mice induced by acetic acid (P <0.05) and Bidens biternata could significantly increase the threshold of pain in mice induced by thermal stimulus. [Conclusions] Radix Helicteris, Thinleaf Adina Fruit, Nervilia fordii, Herba Polygoni Chinensis, Radix Ilicis Asprellae, Trikeraia hookeri, Radix Eupatorii Chinensis, Pronephrium triphyllum, Bidens biternata, Evodia lepta Merr, ovate leaf Holly Bark and Rubus parvifolius had anti-inflammatory action; Radix Helicteris, Nervilia fordii , Herba Polygoni Chinensis, Radix Ilicis Asprellae, Trikeraia hookeri, Pronephrium triphyllum, Bidens biternata, Evodia lepta Merr and ovate leaf Holly Bark, etc. Had analgesic effect.