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从‘巴西’香蕉(Musaacuminata L.AAAgroup‘Brazilian’)根的cDNA文库中获得了一段12-氧-植物二烯酸还原酶OPR(12-oxo-phytodienoic acid reductase)基因的片段,RACE扩增获得全长,命名为MaOPR。该基因全长1512bp,存在一个完整的开放阅读框1287bp,编码429个氨基酸。生物信息学分析表明,该蛋白属稳定蛋白,等电点为8.11,具有一个活性位点,一个基质结合位点,一个黄素单核苷酸结合位点。序列预测分析该蛋白亚细胞定位为细胞质,其不属于跨膜蛋白且不存在信号肽。通过和已知植物的12-氧-植物二烯酸还原酶基因相比,同源性达到66%以上。其中与苜蓿、谷子、粳稻、紫云英的OPR编码的氨基酸序列的同源性均为71%。器官特异性分析表明,MaOPR在香蕉的根、茎、叶片、花和果实中均有所表达,其中在茎和果实中表达量较高。通过对其在ABA抑制剂、乙烯、枯萎病胁迫下的表达结果分析显示,该基因响应以上3种胁迫。
A 12-oxo-phytodienoic acid reductase (OPR) gene fragment was obtained from the cDNA library of the roots of ’Musaacuminata L.AAAgroup’Brazilian’ and amplified by RACE Full-length, named MaOPR. The full length of this gene is 1512bp, there is a complete open reading frame of 1287bp, encoding 429 amino acids. Bioinformatics analysis showed that the protein is a stable protein, the isoelectric point is 8.11, with an active site, a matrix binding site, a flavin mononucleotide binding site. Sequence Prediction Analysis The protein subcellular localization as cytoplasm, which does not belong to the transmembrane protein and absence of signal peptide. The homology is over 66% compared to the 12-oxo-phytadienoate reductase gene from known plants. Among them, the amino acid sequence of OPR encoded by alfalfa, millet, japonica and astragalus was 71%. Organ-specific analysis showed that MaOPR was expressed in banana roots, stems, leaves, flowers and fruits, and higher in stems and fruits. Analysis of the expression results under the stress of ABA inhibitor, ethylene and Fusarium wilt showed that the gene responded to the above three stresses.