乙型肝炎病毒(HBV)前C区第28号密码子的点突变(在1896位碱基G被碱基A代替),可抑制HBeAg的表达,使病毒逃避机体的免疫攻击,这与HBV感染的迁延、传播及暴发型肝炎的发作密切相关。已有检测点突变的各种方法和克隆测序、PCR直接测序、寡核苷酸杂交和RFLP等,但均因存在耗时、操作繁琐等缺点难于推广。 Point mutations in codon 28 of the pre-C region of HBV (in which base G at position 1896 is replaced by a base A) inhibit the expression of HBeAg and allow the virus to evade the body’s immune attack, which is associated with HBV infection Of the delay, spread and the outbreak of fulminant hepatitis are closely related. There are a variety of methods for detecting point mutations and cloning and sequencing, PCR direct sequencing, oligonucleotide hybridization and RFLP, but because of the existence of time-consuming, cumbersome operation and other shortcomings difficult to promote.