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目的:建立HPLC法同时测定草珊瑚中富马酸、异嗪皮啶和迷迭香酸含量。方法:采用Ultimate XB C18(250 mm×4.6 mm,5μm)色谱柱,流动相为乙腈(A)-0.1%磷酸溶液(B),梯度洗脱(0～10 min,2%A→20%A;10～35 min,保持20%A),流速1.0 mL.min-1,检测波长为208 nm,柱温为30℃。结果:富马酸,异嗪皮啶和迷迭香酸的线性范围分别为0.0190～0.133(r=0.9999),0.0204～0.143(r=0.9999),0.0212～0.148μg(r=0.9999);平均回收率(n=6)分别为101.3%(RSD=1.4%),99.1%(RSD=1.1%),98.2%(RSD=1.9%)。结论:该方法简便、快速、准确,适用于草珊瑚中富马酸、异嗪皮啶和迷迭香酸的含量测定。
Objective: To establish a HPLC method for the simultaneous determination of fumaric acid, isopractin and rosmarinic acid in Coral. METHODS: The mobile phase consisted of acetonitrile (A) - 0.1% phosphoric acid (B) and gradient elution (0-10 min, 2% A → 20% A ; 10 ~ 35 min, keep 20% A), the flow rate was 1.0 mL.min-1, the detection wavelength was 208 nm and the column temperature was 30 ℃. Results: The linear range of fumaric acid, isoxabine and rosmarinic acid were 0.0190-0.13 3 (r = 0.9999), 0.0204-0.1431 (r = 0.9999) and 0.0212-0.1488μg (r = 0.9999) Rates (n = 6) were 101.3% (RSD = 1.4%), 99.1% (RSD = 1.1%) and 98.2% (RSD = 1.9%) respectively. Conclusion: The method is simple, rapid and accurate and is suitable for the determination of fumaric acid, isoindoline and rosmarinic acid in the coral.