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本文报告30例外周血干细胞移植,应用细胞集落刺激因子动员干细胞后,采集外周血干细胞进行外周血干细胞移植治疗白血病.男性20例,女性10例,年龄3.7～40岁,急性髓细胞白血病(AML)20例、急性淋巴细胞白血病(ALL)10例,第一次缓解(CR_1)20例,第二次缓解(CR_2)10例.方法:应用动员剂前先联合化疗,待白细胞回升后,采用粒单细胞因子(GM-CSF)动员11例,粒细胞刺激因子(G-CSF)19例、药物剂量3～6μg/kg体重,皮下或静脉注射3～7次,每天1次,在用药后4～7天用血细胞分离机、分高采集2～3次.结果:移植前获得单个核细胞(MNC)数为6.23±2.03×10~8/kg重.GM-CSF数为5.83±0.31×10~4/kg体重CD34~+细胞5.11±0.61×10~6/kg体重(10例).对照组未动员者15例,分离采集5～7次,获得MNC数1.84±0.83×10~8/kg.GM-CSF数为2.73×10~4/kg.造血功能恢复:白细胞>1.0×10~9/kg中位数时间18天(14～46天),血小板>20×10~9/L为21天(14～64天),骨髓增生活跃所需中位时间为36天(18～90),远
This article reports 30 peripheral blood stem cell transplantations. After mobilizing stem cells with CSF, peripheral blood stem cells are collected for peripheral blood stem cell transplantation for leukemia. There are 20 males and 10 females, aged 3.7 to 40 years, acute myeloid leukemia (AML). 20 cases, 10 cases of acute lymphoblastic leukemia (ALL), 20 cases of first remission (CR 1), and 10 cases of second remission (CR 2). METHODS: Combination of chemotherapy before mobilization, after the recovery of leukocytes, Granule monocytokines (GM-CSF) mobilized in 11 cases, granulocyte stimulating factor (G-CSF) in 19 cases, drug dose of 3 to 6 μg/kg body weight, subcutaneous or intravenous injection of 3 to 7 times a day, after administration 4 to 7 days with blood cell separator, the height was collected 2 to 3 times. Results: The number of mononuclear cells (MNC) obtained before transplantation was 6.23±2.03×10~8/kg weight. The number of GM-CSF was 5.83±0.31× 10~4/kg body weight CD34~+ cells 5.11±0.61*10~6/kg body weight (10 cases). Fifteen patients were not mobilized in the control group and collected 5 to 7 times to obtain MNC count 1.84±0.83*10~8 The number of GM-CSF was 2.73×10~4/kg. Recovery of hematopoietic function: WBC>1.0×10~9/kg median time 18 days (14～46 days), platelet >20×10~9/ L is 21 days (14-64 days), bone marrow increases life The median time required for the jump is 36 days (18-90), far