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目的：观察双歧杆菌合剂对急性坏死性胰腺炎（ＡＮＰ）时肠道细菌易位的影响。方法：杂种犬２３只，分３组：对照组（ｎ＝７）仅作剖腹探查，另１６只复制ＡＮＰ模型后，分为微生态调理组（ｎ＝８）和ＡＮＰ组（ｎ＝８）。测定血中胰淀粉酶、内毒素，作脏器细菌培养、病理检查和肠粘膜菌群分析。结果：ＡＮＰ组胰、肠病理损害严重，血淀粉酶（ＡＭＹ）和内毒素（ＬＰＳ）持续升高。肠粘膜大肠杆菌及类杆菌数量增加，双歧杆菌和乳杆菌减少，脏器细菌培养阳性率１００％。与ＡＮＰ组比较，微生态组胰、肠病理损害减轻，肠粘膜类杆菌、大肠杆菌数量减少，双歧杆菌和乳杆菌增加，肠道微生态趋于平衡，脏器中细菌移位率由１００％降为６２．５％，数量减少１０－４０倍，血淀粉酶及内毒素水平下降２～３倍。结论：双歧杆菌合剂可减轻ＡＮＰ后肠粘膜的损害，调整菌群失调，保护肠屏障功能，对防治ＡＮＰ后肠道细菌易位和肠源性感染具有重要作用。
Objective: To observe the effects of bifidobacterium mixture on intestinal bacterial translocation in acute necrotizing pancreatitis (ANP). METHODS: Twenty-three hybrid dogs were divided into 3 groups: control group (n = 7) only for laparotomy; the other 16 rabbits were divided into microbiological conditioning group (n = 8) and ANP group . Determination of blood pancreatic amylase, endotoxin, organ culture, pathological examination and intestinal mucosal flora. Results: The pathological changes of pancreas and intestine in ANP group were serious, and the amylase (AMY) and endotoxin (LPS) were continuously increased. Intestinal mucosal E. coli and Bacteroides increased the number of bifidobacteria and lactobacilli decreased, organ culture positive rate of 100%. Compared with the ANP group, the microbiological group reduced pancreatic and intestinal pathological damage, reduced the number of intestinal mucosal bacilli and E. coli, bifidobacteria and lactobacilli increased, and the gut microflora tended to balance. The bacterial translocation rate in the organism ranged from 100 % Down to 62.5%, the number of reduced 10-40 times, blood amylase and endotoxin levels decreased by 2 to 3 times. Conclusion: Bifidobacterium mixture can relieve the damage of intestinal mucosa after ANP, adjust the flora, protect the intestinal barrier function and play an important role in prevention and treatment of intestinal bacterial translocation and enterogenous infection after ANP.