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选择屏山炒青、霍山黄芽、黄山毛峰、龙井4种非发酵茶和祁门红茶、普洱茶2种发酵茶,分别按1%和2%添加量加入斑点叉尾鮰(Ietalurus punetaus)基础饲料中,进行为期8周的养殖试验。试验结束后,每箱随机捞取7尾试验鱼,测定各组鱼体肝脏组织的谷胱甘肽(GSH)含量、谷胱甘肽过氧化物酶(GSH-Px)、谷胱甘肽还原酶(GR)活性;然后降低水位,对每箱剩余的23尾试验鱼进行3d的拥挤胁迫,再每箱随机捞取7尾测定肝脏组织的3项指标。结果显示:龙井(1%、2%组)在胁迫前可显著提高GSH含量,霍山黄芽(1%、2%组)和祁门红茶(1%组)在胁迫后提高GSH含量的作用更为显著;胁迫前,所有茶叶都不能提高GSH-Px活力;胁迫后,1%屏山炒青、霍山黄芽、祁门红茶组和2%霍山黄芽、龙井组可使GSH-Px活力显著高于对照组;龙井(1%组)在胁迫前能够显著提高GR活力;胁迫后,多数1%组能显著提高GR活力,2%组中,霍山黄芽和龙井组显著高于对照组。结果表明:茶叶可提高GSH含量以及GSH-Px与GR活性,这种提高主要是在胁迫后体现出来,其中霍山黄芽、龙井和祁门红茶的效果更为明显;2种添加量中,1%添加量效果更为明显。
Two kinds of fermented tea such as Panchaoyuqing, Huoshan Huangya, Huangshan Maofeng, Longjing 4 kinds of non-fermented tea and Qimen black tea and Pu’er tea were selected and added into the basal diet of Ietalurus punetaus at 1% and 2% respectively , For a period of eight weeks of breeding trials. At the end of the experiment, seven fish were randomly taken from each box to determine the contents of glutathione (GSH), glutathione peroxidase (GSH-Px), glutathione reductase (GR) activity. Then the water level was lowered. Three of the remaining 23 test fish in each box were crowded for three days. Three indicators of hepatic tissues were randomly collected from each of the seven boxes. The results showed that the content of GSH in Longjing (1%, 2%) group increased significantly before stress, and the effect of Huoshan Huangya (1%, 2%) and Qimen black tea (1% Was significantly higher than that of the control; GSH-Px activity could not be increased by all the tea leaves before stress; GSH-Px activity of 1% Pingshan Chaoqing, Huoshan huangya, Qimen black tea and 2% In the control group, Longjing (1%) significantly increased the activity of GR before stress. After 1% stress, the activity of GR increased significantly in most of the 1% groups, while in Huoshan yellow bud and Longjing group was significantly higher than that in the control group. The results showed that: tea can increase GSH content and GSH-Px and GR activity, which is mainly reflected after the stress, of which Huoshan Huangya, Longjing and Qimen tea more obvious effect; two kinds of additions, 1 % Of the amount of added effect is more obvious.