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目的使用RTnestedPCR分型检测方法及核苷酸序列测定技术,对来源于福建省内HFRS监测点的鼠肺标本及病人血清进行基因分型并对部分标本的核苷酸序列进行分析比较。结果24份阳性鼠肺标本中检出率为95.8%;20份病人血清标本中仅11份扩增阳性,检出率分别为:83%(≤1周),12.5%(>1周)。扩增阳性标本中仅1份RTPCR分型为HTN型,其余均为SEO型,这与核苷酸序列分型结果相一致。结论近年福建省流行的汉坦病毒仍以SEO型为主。核酸序列比较分析发现,福建省内同一地区流行的SEO型汉坦病毒核苷酸的同源性很高,大于99%,而不同地区病毒间核酸序列变异比较大,尤其是永春和松溪这两个地区的毒株差异达到20%左右,可能是SEO型中两个新的亚型。
OBJECTIVE: To genotype rat lung samples and serum from HFRS surveillance sites in Fujian Province using RT-PCR-based genotyping and nucleotide sequencing techniques. Nucleotide sequences of some samples were analyzed and compared. Results The positive rate was 95.8% in 24 positive rat lung samples. Only 11 of 20 positive samples were detected positive in the 20 samples. The detection rates were 83% (≤ 1 week) and 12.5% (> 1 week) respectively. Only 1 RTPCR was genotyped as HTN in the amplified positive samples, and the rest were SEO-type, which was consistent with the results of nucleotide sequence typing. Conclusions In recent years, the prevalence of Hantavirus in Fujian Province is still dominated by SEO type. The comparative analysis of nucleic acid sequences showed that the homology of SEO-typed Hantavirus nucleotides in the same region of Fujian Province is very high, which is more than 99%, while the variation of nucleic acid sequences among different regions is relatively large, especially in Yongchun and Songxi Two strains of the difference between the two regions reached about 20%, may be two new subtypes in the SEO type.