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目的 合成卟啉锰 TFO 吖啶化合物。方法 合成含 7 去氮 2 脱氧黄嘌呤的TFO ;以吖啶修饰TFO的 3′末端 ,以 6碳氨基连接臂修饰TFO的 5′末端 ;四苯基卟啉四羧酸经金属化、酯化后 ,与TFO的 5′末端偶联 ;薄层层析纯化 ,质谱、紫外光光谱分析鉴定。结果 薄层层析结果显示在对照寡核苷酸带前方有一条淡黄色的吖啶 TFO带 ;紫外光光谱分析显示卟啉锰 TFO 吖啶化合物同时具有 2 60nm处寡核苷酸的特征吸收峰和 468nm处卟啉锰的特征吸收峰。质谱分析结果证实 ,卟啉锰 TFO 吖啶化合物分子量实测值与理论值一致。结论 合成了卟啉锰 TFO 吖啶化合物
Aim To synthesize porphyrin manganese TFO acridine compounds. Methods TFO containing 7 deaza 2 deoxyxanthines was synthesized. The 3 ’end of TFO was modified by acridine and the 5’ end of TFO was modified by 6 carbon amino linker. The tetraphenylporphyrin tetracarboxylic acid was metallized and esterified After coupling with the 5 ’end of TFO, TLC, purification and identification by UV-Vis and MS were carried out. Results TLC showed that there was a pale yellow acridine TFO band in front of the control oligonucleotide. UV spectroscopy showed that the porphyrin manganese TFO acridine compound also had the characteristic absorption peak at 260 nm And the characteristic absorption peak of porphyrin manganese at 468nm. Mass spectrometry results confirmed that the measured molecular weight of the porphyrin manganese TFO acridine compound is consistent with the theoretical value. Conclusion The porphyrin manganese TFO acridine compounds were synthesized