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试验母牛选自22个最近有钩端螺旋体病,或有流产现象的牛群。每头试验母牛在11个月内采集多次血和尿的样品。用显微镜和培养方法检查钩端螺旋体尿。用波摩那(Pomona)、哈吉欧(hardjo)、考贲哈纪尼(Copenhageni)和拜伦血清型肾脏钩钩端螺旋体作抗原,以半自动补体结合试验和显微镜凝集试验检查血清。107头母牛检查出钩端螺旋体尿。其中43头母牛培养出波摩那型、13头母牛培养出哈吉欧型钩体。其余51头母牛未鉴定出该病原的种类。与波摩那型和哈吉欧型钩体抗原,具有最高的补体结合试验和显微镜凝集试验效价。没有发现考贲哈纪尼型或拜伦型钩体感染的细菌学或血清学变化。有钩端螺旋体尿的母牛,其补体结合试验的反应数与显微镜凝集试验的反应数没有显著差异。没有钩端螺旋体尿的母牛,很少出现补体结合反应,并对大多数病例,补体结合抗体的检出时间比凝集素短。接近流产时,有钩端螺旋体尿的流产母牛,对这两种试验均出现反应。我们的试验结果表明,补体结合试验在动物健康检验室中,对牛钩端螺旋体病的血清学诊断是有效的。
The test cow was selected from 22 herds with recent leptospirosis or abortions. Each trial cow collected samples of blood and urine multiple times within 11 months. Leptospira urine was examined by microscopy and culture. Pomona, hardjo, Copenhageni and Byron’s serotypes of L. reniformis were used as antigens, and sera were examined using a semi-automatic complement fixation assay and a microscope agglutination test. 107 cows checked for leptospirosis urine. Of these, 43 were bovine and 13 were bred to produce Hajiou. The remaining 51 cows did not identify the species of the pathogen. And Pomona and Hajiou type hook antigen, with the highest complement fixation test and microscope agglutination test titer. No bacteriological or serological changes were found in the Kobakihajini or Byron leptospirosis. There was no significant difference in the number of reactions in the complement fixation test and in the microscopic agglutination test with leptospira urine. In cows without Leptospira urine, there is very little evidence of complement fixation and, in most cases, complement fixation antibodies are detected shorter than lectins. Near miscarriage, abortive cows with Leptospira urine reacted to both tests. Our results show that the complement fixation assay is effective in the serological diagnosis of leptospirosis in the animal health laboratory.