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迄今人们借助于Y染色体特异性探针的原位杂交技术已能对胚胎(体外受精)进行性别鉴定。但由于Y染色体的标记探针的显示需一定时间,故组织活检的胚胎须冷冻直至胚胎性别的诊断确定,因此有可能对胚胎造成损伤。最近,Winston和Handyside等利用多聚酶链反应(polymerase chain reaction,PCR)技术,通过对特定的DNA序列的扩增从而识别Y染色体,使人胚胎的性别鉴定更为可靠和迅速。Winston等在卵子打洞进行人工授精后的第3天即受精卵发育至6～10个细胞时,取其中一个细胞进行鉴定。经组织活检的胚胎发育,存活看来并不
So far it has been possible to characterize embryos (in vitro fertilization) by means of in situ hybridization with Y chromosome-specific probes. However, due to the Y chromosome labeled probe display takes time, so the biopsy embryos to be frozen until the embryo sex determination diagnosis, it may cause damage to the embryo. Recently, Winston and Handyside et al. Used polymerase chain reaction (PCR) to identify the Y chromosome by amplifying a specific DNA sequence, making sex determination of human embryos more reliable and rapid. Winston and other eggs in the hole after artificial insemination on the 3rd day after the fertilized egg development to 6 to 10 cells, whichever one cell identification. Tissue biopsy embryo development, survival does not seem