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目的比较不同采样方法对腹腔镜器械灭菌监测结果的影响。方法采用活菌计数检测方法,对4种采样法比较腹腔镜器械灭菌效果。结果腹腔镜分离钳轴关节和咬合齿单独采用涂擦法采样,阳性率为2.78%,而单独采用冲洗法采样,细菌培养全部为阴性结果;采用浸泡+反复冲洗法采样,细菌培养阳性率为16.67%。经统计学2х检验,两组涂擦法采样结果之间差异无统计学意义;冲洗法与浸泡+反复冲洗法的监测结果之间差异有统计学意义(P值<0.05)。结论采用浸泡+反复冲洗法采样,监测结果能比较客观,具有良好的可操作性。
Objective To compare the effects of different sampling methods on the results of laparoscopic sterilization monitoring. Methods The viable count method was used to compare the sterilization effects of laparoscopic instruments on four sampling methods. Results Laparoscopic separation forceps shaft and joint teeth were smeared separately with a smear method, the positive rate was 2.78%, while the method of flushing alone was used for sampling, bacterial culture was all negative results; the sample was soaked + repeated washing, the positive rate of bacterial culture was 16.67%. There was no statistically significant difference between the two smear smear sampling results by the statistical 2 × test. The difference between the flushing and immersion + repeated flushing methods was statistically significant (P <0.05). Conclusion The sample is soaked + repeated washing, the monitoring results can be more objective, with good maneuverability.