论文部分内容阅读
以成熟胚愈伤组织为转化受体 ,经农杆菌介导法将显性广谱白叶枯病抗性基因 Xa2 1导入高感白叶枯病的水稻广亲和恢复系 SWR2 0中 ,共获得 4 3个独立的转基因株系 ,1 0 0余株转基因水稻植株。经 GUS活性、潮霉素抗性检测及PCR分析表明 ,外源基因已整合到转基因水稻基因组中 ;白叶枯病原菌接种试验表明 ,大部分 T0 代转基因水稻植株的抗病性有明显提高 ,多数植株表现抗或高抗 ,个别为感病。经对农杆菌介导的水稻转化技术作进一步改进后 ,GUS瞬间表达率可高达 92 .5% ,稳定转化频率达到 2 7.6%
Mediated by Agrobacterium tumefaciens, the dominant broad-spectrum bacterial leaf blight resistance gene Xa21 was introduced into SWR20, a plant wide-compatibility restorer line with high susceptibility to bacterial leaf blight 43 independent transgenic lines and more than 100 transgenic rice plants were obtained. GUS activity, hygromycin resistance test and PCR analysis showed that exogenous genes have been integrated into the genome of transgenic rice. Bacterial blight inoculation tests showed that most of the T0 transgenic rice plants have significantly increased disease resistance, the majority Plants show resistance or high resistance, some are susceptible. After further improvement of Agrobacterium-mediated rice transformation, the transient expression rate of GUS could reach as high as 92.5% and the stable transformation frequency reached 7.66%