目的　掌握人子宫平滑肌细胞原代培养的方法 ,可以籍之开展一系列的研究工作。比如进行体外药物实验 ,研究细胞的增殖与遗传 ,了解平滑肌细胞的动力学效应等许多方面。而且该方法目前在国内未见成功报道。方法　用剪切胶原酶消化法分离人子宫平滑肌细胞。用DMEM和胎牛血清进行培养。传一代时用desmin和α -action单抗进行鉴定。结果　经实验该法成功率高 ,纯度高 ,细胞活力好。结论　用该法可以成功进行人子宫平滑肌细胞的原代培养 Objective To master the method of primary culture of human uterine smooth muscle cells, and to carry out a series of research work. For example, in vitro drug experiments to study cell proliferation and inheritance, to understand the kinetic effects of smooth muscle cells and many other aspects. And this method has not been successfully reported in China at present. Methods Human uterine smooth muscle cells were isolated by shear collagenase digestion. Culture with DMEM and fetal bovine serum. The passage of generation with desmin and α-act MAb identification. The results of the experiment by the method of high success rate, high purity, good cell viability. Conclusion This method can successfully carry out the primary culture of human uterine smooth muscle cells