论文部分内容阅读
本文介绍用10个马铃薯品种的顶端分生组织培养的试管植株,经过切段繁殖并在人工控制条件下诱导产生气生块茎的方法。研究外源激素、蔗糖浓度、温度和光照等外界因素对试管植株产生块茎的影响。结果表明:低温低光照强度和培养基中高浓度蔗糖有利于气生块茎的发育和生长。高温、长光照及培养基中低浓度蔗糖不利于小块茎的产生。培养基中高浓度蔗糖加上激素具有促进气生块茎形成的作用。而且,气生块茎的形成与品种的基因型有关,其产生的条件,各品种间存在明显的差异。因此马铃薯离体植株气生块茎的产生是受植物体内外各因素综合影响的结果。
This article describes a method of in vitro culture of test tube plants from the top of 10 potato cultivars, propagated by segmented breeding and induced to produce aerial tubers under controlled conditions. The effects of external factors such as exogenous hormones, sucrose concentration, temperature and light on tuber production in vitro were studied. The results showed that the low temperature and low light intensity and high concentration of sucrose in the medium were conducive to the development and growth of aerial blight. High temperature, long light and medium low concentrations of sucrose is not conducive to the production of small tubers. High concentrations of sucrose in the medium plus hormones have the effect of promoting aerial tuber formation. Moreover, the formation of air-dried tubers is related to the genotypes of the cultivars, and the conditions for their production are obviously different among the cultivars. Therefore, the production of aerial tubers of potato plants in vitro is a result of the combined effect of various factors inside and outside plants.