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为探讨人类免疫缺陷病毒1型HIV-1 gp120基因多样性和生物学活性位点与艾滋病痴呆综合征之间的关系,从一例艾滋病痴呆综合征病例尸检标本的淋巴结和中枢神经组织(大脑5个部位:颞叶灰/白质连接处、脑室周围组织、脉络丛、枕叶白质及枕叶灰/白质连接处)提取不同组织来源的基因组DNA,经PCR法扩增HIV-1 gp120基因,经克隆后挑选阳性克隆菌株,对插入片段进行测序。用生物学软件处理并绘制系统发生树,分析糖基化位点,计算ds/dn值,分析V3顶端四肽及关键位点的氨基酸。结果显示,该病人感染的病毒是HIV-1B亚型;分离自不同组织的HIV-1 gp120基因存在差异;与标准序列相比,分离自该病人的HIV-1 gp120基因的部分生物学活性位点存在改变,且源自外周淋巴组织与中枢神经组织的HIV-1 gp120基因中部分生物学活性位点也存在差异。结果表明,HIV-1 gp120基因多样性及与脑组织相关的某些生物学活性位点的改变可能与艾滋病痴呆综合征的发病机制存在一定关系。
To investigate the relationship between HIV-1 gp120 genotypes and biological active sites of human immunodeficiency virus type 1 HIV-1 and AIDS dementia syndrome, from an autopsy specimen of AIDS dementia syndrome in lymph nodes and central nervous system (5 parts of the brain : The temporal lobe gray / white matter junction, periventricular tissue, choroid plexus, occipital lobe white matter and occipital lobe gray / white matter junction) extracted from different tissues of genomic DNA, PCR amplified gp120 HIV-1 gene after cloning Positive clones were selected and the inserts were sequenced. Phylogenetic trees were processed and mapped using biological software, glycosylation sites were analyzed, ds / dn values calculated, amino acids at the top tetrapeptide V3 and key sites analyzed. The results showed that the HIV-1 subtype was infected by this patient; the HIV-1 gp120 gene isolated from different tissues was different; some biologically active sites of HIV-1 gp120 gene isolated from this patient There was also a change in the spots, and there were also differences in some biologically active sites in the HIV-1 gp120 gene derived from peripheral lymphoid tissues and central nervous tissues. The results showed that the diversity of HIV-1 gp120 gene and some biological active sites related to brain tissue may be related to the pathogenesis of AIDS dementia syndrome.