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以ＨＣＶ－ＵＳ为原型并根据台湾株ＨＣＶ－Ｔ３序列加以修正，设计出呈巢式排列的ＨＣＶ基因引物，结合由特异基因扩增产物经缺口翻译而制成的ＨＣＶｃＤＮＡ探针Ｓｏｕｔｈ－ｅｒｎ转印杂交，建立检测ＨＣＶＲＮＡ的逆转录多聚酶链反应技术，该技术操作相对简便，特异性和灵敏度高。应用这一技术检测５４例慢性ＮＡＮＢＨ患者血浆ＨＣＶＲＮＡ，阳性３４例，占６３．０％，若考虑抗ＨＣＶ阳性例数，共计检出ＨＣＶ标记物阳性４４例，达８１．５％，表明ＨＣＶ为ＮＡＮＢＨ的主要致病原；同时检测４９例ＨＢｓＡｇ阳性的慢性肝病病人，血浆ＨＣＶＲＮＡ阳性１４例，占２８．６％，ＨＢＶ／ＨＣＶ重叠感染１８例，双重感染率高达３６．７％
HCV-US was used as a prototype and was modified according to the Taiwan strain HCV-T3 sequence to design a nested HCV gene primer. The HCV cDNA probe South-ern made by nicking the specific gene amplification product was transferred Hybridization, the establishment of reverse transcriptase polymerase chain reaction detection of HCVRNA, the technology is relatively simple, high specificity and sensitivity. HCV RNA was detected in 54 patients with chronic NANBH using this technique, 34 cases were positive, accounting for 63.0%. If we consider the number of anti-HCV positive cases, a total of 44 HCV positive samples (81.5%) were detected, indicating that HCV NANBH the main pathogen; simultaneous detection of 49 cases of HBsAg-positive chronic liver disease patients, plasma HCVRNA-positive in 14 cases, accounting for 28.6%, HBV / HCV overlap infection in 18 cases, the double infection rate as high as 36.7%