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用电导法测定了小麦叶片经Gn-粗毒素处理后的组织细胞膜损伤情况,发现毒素能导致小麦叶片细胞内电解质外渗,不同抗性品种对毒素反应有明显差异,抗病品种的细胞损伤率明显低于感病品种。电镜观察发现,毒素还引起组织超微结构发生显著变化,包括叶绿体膜上出现电子致密度高的沉积物,基粒片层肿胀,排列紊乱;直至叶绿体、细胞核和整个细胞解体。损害发生最早最严重的是叶绿体基粒片层。抗病品种比感病品种的超微结构受害轻、反应迟。
The electrical conductivity method was used to determine the cell membrane damage of wheat leaves after Gn-crude toxin treatment. It was found that toxin could cause extracellular electrolyte leakage in wheat leaves. The response of different resistant varieties to toxins was significantly different. The cell damage rate Significantly lower than susceptible varieties. Electron microscopy showed that the toxin also caused significant changes in the ultrastructure of the tissue, including the deposition of electron dense densities on the chloroplast membrane, swollen and disorganized grana lamellae until the chloroplast, nucleus and whole cell disintegrated. The earliest and most damaging damage is the chloroplast grana. Disease resistant varieties of susceptible varieties than the ultrastructure of victims of light, late response.