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目的:探讨毛冬青总黄酮对大鼠局灶脑缺血再灌注模型的作用及其机制。方法:大鼠灌服不同剂量毛冬青总黄酮5 d,5 d给药后1 h分离大鼠左侧颈总动脉(common carotid artery,CCA)、颈外动脉(external ca rotid artery,ECA)、颈内动脉(internal carotid artery,ICA)。采用线栓法,缺血2 h,再灌注24 h,复制局灶脑缺血再灌注模型;神经症状评分后,采用血清放免法,检测白细胞介素-1β(interleukin-1β,IL-1β)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α);取大鼠脑组织匀浆,测超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malonaldehyde,MDA)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)含量;另一半脑组织固定、切片,观察脑组织病理变化及脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)、血管内皮生长因子(vascular endothelial growth factor,VEGF)表达情况。结果:采用线栓法成功复制大鼠局灶脑缺血再灌注模型;与模型相比,毛冬青总黄酮大剂量能显著降低IL-1β、TNF-α含量,升高SOD、GSH-Px含量,降低MDA含量,提升BDNF、VEGF阳性表达(P<0.01,P<0.05)。结论:毛冬青总黄酮可显著改善大鼠局灶脑缺血再灌注模型的脑缺血损伤。
Objective: To investigate the effect of Mao Dongqing total flavone on focal cerebral ischemia-reperfusion in rats and its mechanism. Methods: The rats were drenched with different doses of total flavonoids of Mao Dongqing for 5 days, and the left common carotid artery (CCA), external carotid artery (ECA) Internal carotid artery (ICA). The model of focal cerebral ischemia-reperfusion was established by the method of thread embolism, 2 h ischemia and 24 h reperfusion. Serum radioimmunoassay was used to detect the expression of interleukin-1β (IL-1β) , Tumor necrosis factor-α (TNF-α) were measured. The contents of superoxide dismutase (SOD), malonaldehyde (MDA) The contents of glutathione peroxidase (GSH-Px) in the other half of brain tissue were fixed and sectioned to observe the pathological changes of brain tissue and the changes of brain-derived neurotrophic factor (BDNF), vascular endothelial growth factor (vascular endothelial growth factor, VEGF) expression. Results: The rat model of focal cerebral ischemia-reperfusion was successfully established by the method of thread embolization. Compared with the model, the high dose of the total flavonoids of Mao dongqing could significantly decrease the contents of IL-1β and TNF-α and the content of SOD and GSH-Px , Decreased MDA content, increased BDNF and VEGF expression (P <0.01, P <0.05). Conclusion: Total flavonoids from Mao dongqing can significantly improve cerebral ischemia injury in focal cerebral ischemia-reperfusion model in rats.