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目的将尼龙绢集卵法与改良加藤厚涂片法(简称加藤法)相结合加以改进,进行实验室预试验和现场研究。方法实验室设计制作EPG(每克粪虫卵数)分别为4、8、16、24、48、96六种阳性粪便标本,现场选定低、中、高三个不同感染率的流行村各粪检居民200人,对各EPG组和每人份粪便按30g、5g、41.7mg粪量分组,分别用改进法和加藤法作粪检,进行阳性检出率和EPG值的比较。结果改进法两组阳性检出率均高于加藤法,EPG在4时,加藤法未能检出,漏检率100%,EPG在24及其以上(新鲜血吸虫卵)两法检出率相等,检出EPG值相近。低度流行村,加藤法未能查出病人,改进法两组均可查出,且30g组优于5g组。中、高度流行村,加藤法检出阳性率渐增,但仍低于改进法;检出阳性病人EPG值,改进法的30g组与加藤法相近。结论EPG在24以下及低度流行区不宜用加藤法查病,采用改进法较好,EPG在24以上和中、高度流行区查病,两种方法均可采用。
OBJECTIVE To improve the combination of nylon silk spawning method and modified Kato thick smear method (Kato method) for laboratory pre-test and field research. Methods The laboratories designed and produced six positive stool specimens of EPG (number of eggs per gram) of 4, 8, 16, 24, 48 and 96 respectively. Three low, medium and high prevalence villages 200 residents were inspected, and the faeces of each EPG group and each person were grouped according to the amount of 30g, 5g and 41.7mg, respectively. The positive rate and the value of EPG were compared with improved method and Kato method respectively. Results The positive detection rate of the improved method was higher than that of Kato. When the EPG was at 4 o’clock, the Kato method failed to detect, the missed detection rate was 100%, and the detection rate of two methods of EPG at 24 and above (fresh schistosome eggs) was equal , Similar EPG values were detected. Low prevalence village, Kato method failed to detect the patient, improved method can be found in both groups, and 30g group is better than the 5g group. Medium and high prevalence village, Kato method was detected positive rate increased, but still lower than the improved method; detected positive patients EPG value, improved method 30g group and Kato method similar. Conclusions EPG should not be used for Kocha disease in less than 24 and low prevalence areas. It is better to use the improved method, EPG is more than 24 and moderate and high prevalence area, both methods can be used.