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作者不久前建立了应用RNA病毒——脊髓灰质炎病毒(PV)作重组疫苗载体的系统。构建的PV基因组中核壳区被人类免疫缺陷症病毒(HIV)gag和env基因片段所取代。由重组痘苗病毒(VV-P1)反式提供核壳蛋白的互补系统使缺损的基因组能被核壳包裹。用这类复制子感染细胞可表达与PV核壳蛋白PV4和PV1融合的重组蛋白。 本研究中,作者采用了表达HIV-1 gag核壳蛋白(p24)以及HIV-11.5kb env基因的有核壳包裹的复制子。这种复制子是在VV-P1存在的情况下经20次连续传代获得的。将这种有核壳包裹的复制子在2型PVLansing株存在的情况下传代(共感染)可使复制子被PV核壳蛋白所包裹。这种类型的复制子至少经三次连续传代后仍然存在。将这种表达HIV-1 gag、被2型PV Lansing株核壳包裹的复制子经肌肉、直肠或胃途径接
The author recently established a system that uses the RNA virus, the poliovirus (PV), as a recombinant vaccine vector. The nucleocapsid region of the constructed PV genome was replaced with the human immunodeficiency virus (HIV) gag and env gene fragments. Complementary systems of nucleocapsid proteins trans-expressed by the recombinant vaccinia virus (VV-P1) allow the defective genome to be enveloped by the nucleocapsid. Infection of cells with such replicons can express recombinant proteins fused to the PV nucleocapsid proteins PV4 and PV1. In this study, we used a nucleocapsid-replicon replicator expressing the HIV-1 gag nucleocapsid protein (p24) and the HIV-11.5 kb env gene. This replicon was obtained after 20 consecutive passages in the presence of VV-P1. Replicating this nucleocapsid-coated replicon in the presence of type 2 PVLansing strain (co-infection) allows the replicon to be enveloped by the PV nucleocapsid protein. This type of replicon still persists after at least three successive passages. The HIV-1 gag, which is replicated in the nucleocapsid vesicles of type 2 PV Lansing strain, is administered via the muscle, rectum or stomach